Vero-Hektor

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: African green monkey kidney
• Growth mode: Adherent
• Karyotype: Not Specified
• Morphology: Fibroblast-like

Właściwości

• biological source: African green monkey kidney
• packaging: tube of 5 μg 03092503-DNA-5UG, pkg of vial of cells 03092503-1VL
• growth mode: Adherent
• karyotype: Not Specified
• morphology: Fibroblast-like
• receptors: Not specified

Opis

Biochem/physiol Actions

African green monkey kidney cells, serum-free

Vero cell line (Sigma catalog no. 84113001) adapted to grow in the chemically defined protein- and peptide-free medium Hektor G (Cell Culture Technologies). This cell line is substrate dependent, i.e., adherent and may be suitable for the replication of viral particles.

Preparation Note

Hektor G medium (Cell Culture Technologies http://www.cellculture.com/) + 4 mM L-Glutamine (G7513). When freezing cells down use Hektor G medium + 4 mM L-Glutamine (G7513) + 10% DMSO (D2650).

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm². Use EDTA only (no trypsin); 5% CO₂; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].

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