C3115

Control Primer Set for Arabidopsis CBP20 Gene

• UNSPSC Code: 12352200

Właściwości

• storage temp.: −20°C

Opis

General description

This product consists of a primer set for the Arabidopsis housekeeping gene CBP20 (At5g44200). CBP20 encodes a nuclear cap-binding protein. It is intended for use as an internal control for Q-RT-PCR for expression analysis of any Arabidopsis gene.

Of our three control primer series for Arabidopsis gene expression, the CBP20 control primer is designed as a control for medium- to low-abundant gene expression. The other two control primers in the series are ubiquitin (UBC, Product Code C3240) also designed as a control for medium- to low-abundant gene expression and actin-2 (Product Code C3615) for high-abundant gene expression.

Application

Primer should be stored at -20 °C. Primers are stable at -20 °C for at least 1 year and can be frozen and thawed at least 10 times.

Other Notes

Each tube contains enough primer (forward or reverse) for 100 RT-PCR reactions (50 μl reaction volume)

• Forward Primer (T_m = 68 °C based on % GC method in 1 M salt), 20 μM, in TE buffer, Product Code C2740.
• Reverse Primer (T_m = 66 °C based on % GC method in 1 M salt), 20 μM, in TE buffer, Product Code C2865.

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