B5525

Bromophenol Blue sodium salt

• Synonim(y): 3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt
• Wzór empiryczny (zapis Hilla): C₁₉H₉Br₄NaO₅S
• Numer CAS: 34725-61-6
• Masa cząsteczkowa: 691.94
• UNSPSC Code: 12171500
• NACRES: NA.52
• PubChem Substance ID: 57653941
• EC Number: 252-170-2
• Beilstein/REAXYS Number: 6995701
• MDL number: MFCD00013793

Właściwości

• form: powder
• Quality Segment: 200
• technique(s): electrophoresis: suitable
• solubility: water: soluble
• suitability: suitable for nucleic acid electrophoresis
• storage temp.: room temp
• SMILES string: [Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O
• InChI: 1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1
• InChI key: TWKDJMRHSLWRFI-UHFFFAOYSA-M

Opis

General description

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis.[1] It has also been used in Drosphila fly food to aid visualization of gut for dissection.[2]

Preparation Note

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)