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Merck

AA0100

Ammonia Assay Kit

sufficient for 100 assays

Synonim(y):

Ammonia Quantitative Test Kit

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1 KIT

1750,00 zł

1750,00 zł

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Informacje o tej pozycji

NACRES:
NA.84
UNSPSC Code:
12352202

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usage

sufficient for 100 assays

storage temp.

2-8°C

Quality Level

200

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Superoxide Anion Assay Kit

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

usage

sufficient for 100 assays

usage

sufficient for 50 assays

usage

sufficient for 100 assays

usage

sufficient for 100 assays (96 well plates)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Application

Ammonia concentration was measured in serum and urine using the Sigma Ammonia Assay Kit in a study assessing expression of renal ammonia transporters in response to chromic metabolic acidosis. [1]
This kit is for the quantitative, enzymatic determination of ammonia in food and biological samples. Ammonia reacts with α-ketoglutaric acid (KGA) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) in the presence of L-glutamate dehydrogenase (GlDH) to form L-glutamate and oxidized nicotinamide adenine dinucleotide phosphate (NADP+). The decrease in absorbance at 340 nm, due to the oxidation of NADPH, is proportional to the ammonia concentration. L-Glutamate dehydrogenase reacts specifically with ammonia. The Ammonia Assay Kit may be used to determine ammonia concentrations in the range of 0.2-15 μg/ml.
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Tylko elementy zestawu

Numer produktu
Opis
  • Ammonia Assay Reagent 10 vial(s)
  • L-Glutamate Dehydrogenase for Ammonia Assay 1 mL/vial
  • Ammonia Standard Solution 1 mL/vial

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H319,H334

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1

Klasa składowania

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000463998
0000463998
0000369917
0000369917
0000401692

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Odwiedź Bibliotekę dokumentów

Junpeng Jiang et al.
BMC plant biology, 19(1), 239-239 (2019-06-07)
Ammonium transporters (AMTs), a family of proteins transporting ammonium salt and its analogues, have been studied in many aspects. Although numerous studies have found that ammonium affects the interaction between plants and pathogens, the role of AMTs remains largely unknown
Tingting Xiang et al.
Nature communications, 11(1), 108-108 (2020-01-09)
In cnidarian-Symbiodiniaceae symbioses, algal endosymbiont population control within the host is needed to sustain a symbiotic relationship. However, the molecular mechanisms that underlie such population control are unclear. Here we show that a cnidarian host uses nitrogen limitation as a
Renal expression of the ammonia transporters, Rhbg and Rhcg, in response to chronic metabolic acidosis.
Seshadri, R.
American Journal of Physiology: Renal Physiology, 290, F397-F408 (2006)
Bergmeyer, H.U., and Beutler, H-O.
Methods of Enzymatic Analysis, 454-461 (1985)
W E Neeley et al.
Clinical chemistry, 34(9), 1868-1869 (1988-09-01)
This is an automated, cost-efficient enzymatic method for determining ammonia in plasma. In most assays for ammonia the reagents are stable for only one or two days at 4 degrees C, but the reagents in our method are stable for
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Questions

1–10 of 10 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Could you please confirm whether this kit is suitable for quantifying ammonia levels in plant tissues, particularly in roots and shoots?"

    1 answer

    1. This kit has not been tested for its suitability for the detection of ammonia concentration in plant tissues.

      Helpful?

  3. How do I make the standard curve?

    1 answer

    1. A standard curve is not required for this kit. The Ammonia Standard Solution should be included with each set of assays. If the value obtained with the Ammonia Standard Solution is within 5% of the stated concentration, the test performance is acceptable and sample data is valid.

      Helpful?

  4. Can we use this kit for microplate reader system

    1 answer

    1. The protocol below has not been tested but is based on adaptations of the reagents to similar instruments.
      In order to perform the assay in a microplate instead of cuvettes, the reagent and sample volumes can be reduced by a factor of 5. Thus, the reagent volume would be 200 microliters; the volume of water or sample would be 20 microliters, and the volume of standard would be 10 microliters. The required volume of the enzyme solution would decrease to 2 microliters, which is difficult to pipette accurately. To deliver the enzyme more accurately, dilute it 5x P5244 using Phosphate Buffer, pH 7.5, as the enzyme diluent and pipette 10 microliters into each well.

      Helpful?

  5. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  6. In the case of bacterial culture supernatant (BHI broth), should the decolorization process be performed? How?

    1 answer

    1. Any sample solution that is strongly colored and/or has a low ammonia concentration should be decolorized when using this kit. Colored sample solutions often are treated with decolorizing carbon (activated charcoal) to remove color-contributing impurities.

      Helpful?

  7. Is there a control that can be used to assess the accuracy of Product AA0100, Ammonia Assay Kit?

    1 answer

    1. A convenient control for use with this kit is the Ammonia Standard Solution that is provided in the kit.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. How can I assess the absorbance change of my reagent blank when using Product AA0100, Ammonia Assay Kit?

    1 answer

    1. Generally, an absorbance change of 0.050 or less indicates an acceptable range for the reagent blank. An absorbance change higher than this indicates possible contamination of the reagent (or glassware) with ammonia.

      Helpful?

  10. Which samples should be deproteinized for use with Product AA0100, Ammonia Assay Kit?

    1 answer

    1. The basis for this kit was a Diagnostic procedure for the determination of Ammonia in human plasma, whose total protein concentration is approximately 63 to 83 mg/mL. Thus, any solution whose protein concentration is less than approximately 83 mg/mL should not require deproteinization.

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