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Dokumenty

A9169

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

Synonim(y):

Anti Rabbit Antibody - Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat, Anti Rabbit Antibody Sigma

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About This Item

Numer MDL:
MFCD00162788
Kod UNSPSC:
12352203
NACRES:
NA.46

pochodzenie biologiczne

goat

białko sprzężone

peroxidase conjugate

forma przeciwciała

IgG fraction of antiserum

rodzaj przeciwciała

secondary antibodies

klon

polyclonal

Postać

buffered aqueous solution

reaktywność gatunkowa

rabbit

metody

direct ELISA: 1:50,000 using using 5 μg/ml of rabbit IgG for coating and OPD substrate
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
western blot (chemiluminescent): 1:80,000-1:160,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per well)

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

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Opis ogólny

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge.
Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody binds all rabbit Igs.

Specyficzność

Specificity of the Peroxidase Conjugated Anti-Rabbit IgG antibodies is determined by immunoelectrophoresis (IEP) versus normal rabbit serum and rabbit IgG.

Immunogen

purified rabbit IgG

Zastosowanie

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody has been used in western blotting.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody can be used for immunoblot assays at 1:1,000. The antibody can also be used for ELISA (1:5,000) and IHC (1:400) applications.
Cell-associated protein samples from E. coli cultures were analyzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody.
Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.

Działania biochem./fizjol.

Immunoglobulins are glycoprotein antibodies that modulate several immune responses. Immunoglobulin G participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Uwaga dotycząca przygotowania

Prepared by two-step glutaraldehyde method.

Przechowywanie i stabilność

For continuous use, store at 0-5EC. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Piktogramy

Health hazard
GHS08

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H317,H334

Zwroty wskazujące środki ostrożności

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Klasyfikacja zagrożeń

Resp. Sens. 1 - Skin Sens. 1

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Odwiedź Bibliotekę dokumentów

Expression of GABA A α2-, β1-and γ-receptors are altered significantly in the lateral cerebellum of subjects with schizophrenia, major depression and bipolar disorder.
Fatemi SH, et al.
Translational Psychiatry, 3(9), e303-e303 (2013)
Sarah J Coulthurst et al.
The Journal of biological chemistry, 283(35), 23739-23753 (2008-06-20)
Erwinia carotovora subsp. atroseptica is an enterobacterial phytopathogen causing economically significant soft rot disease. Pathogenesis is mediated by multiple secreted virulence factors, many of which are secreted by the type II (Out) secretion system. DsbA catalyzes the introduction of disulfide
L J M Carvalho et al.
Scandinavian journal of immunology, 59(4), 363-372 (2004-03-31)
The immunogenicity and protective efficacy of various antigen-adjuvant formulations derived either from the merozoite-surface protein-3 (MSP-3) or the glutamate-rich protein (GLURP) of Plasmodium falciparum were evaluated in Saimiri sciureus monkeys. These proteins were selected for immunogenicity studies based primarily on
MDR1-deficient genotype in Collie dogs hypersensitive to the P-glycoprotein substrate ivermectin.
Roulet A, et al.
European Journal of Pharmacology, 460(2-3), 85-91 (2003)
Deficits in GABAB receptor system in schizophrenia and mood disorders: a postmortem study.
Fatemi SH, et al.
Schizophrenia Research, 128(1-3), 37-43 (2011)
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