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Merck

A6154

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonim(y):

Goat Anti-Rabbit IgG (whole molecule)–HRP

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About This Item

Numer MDL:
Kod UNSPSC:
12352203
NACRES:
NA.46

pochodzenie biologiczne

goat

białko sprzężone

peroxidase conjugate

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

secondary antibodies

klon

polyclonal

Postać

buffered aqueous solution

reaktywność gatunkowa

rabbit

metody

direct ELISA: 1:10,000

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

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Opis ogólny

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody is immunospecific for rabbit IgG by immunoelectrophoresis against normal serum and rabbit IgG, prior to conjugation.

Immunogen

purified rabbit IgG

Zastosowanie

C33A cell lysates were analyzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. Protein aliquots from colonic mucosal extracts (μg) were anlayzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody at a 1:5000 dilution in TBSt for 1 hr at room temperature.
Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody has been used for western blot (1:6,000), and ELISA assays.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Uwaga dotycząca przygotowania

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Piktogramy

Exclamation markEnvironment

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Odwiedź Bibliotekę dokumentów

Johan de Vogel et al.
Carcinogenesis, 29(2), 398-403 (2008-01-05)
Epidemiological and animal model studies suggest that a high intake of heme, present in red meat, is associated with an increased risk of colon cancer. The aim of this study was to elucidate the effects of dietary heme on colonic
Pascal Nurwakagari et al.
Journal of molecular endocrinology, 38(1-2), 259-275 (2007-02-13)
Glycoprotein hormone receptors such as the lutropin/chorionic gonadotropin receptor (LHR) are characterized by a large N-terminal ectodomain (ECD), which is responsible for hormone-receptor interactions. For the closely related TSH receptor (TSHR), it has been proposed that the ECD also serves
Patrick Eldin et al.
Nucleic acids research, 42(3), 1698-1710 (2013-11-02)
The human immunodeficiency virus type 1 (HIV-1) Vpr protein binds to the cellular uracil-DNA glycosylase UNG2 and induces its degradation through the assembly with the DDB1-CUL4 ubiquitin ligase complex. This interaction counteracts the antiviral activity exerted by UNG2 on HIV-1
Sandra V Ashton et al.
Arteriosclerosis, thrombosis, and vascular biology, 25(1), 102-108 (2004-10-23)
Invasion of uterine spiral arteries by extravillous trophoblasts in the first trimester of pregnancy results in loss of endothelial and musculoelastic layers. This remodeling is crucial for an adequate blood supply to the fetus with a failure to remodel implicated
A Moles et al.
Oncogene, 35(35), 4623-4632 (2016-02-09)
Post-translational modifications of nuclear factor (NF)-κB subunits provide a mechanism to differentially regulate their activity in response to the many stimuli that induce this pathway. However, the physiological significance of these modifications is largely unknown, and it remains unclear if

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