Agaroza z 3′,5′-cyklicznym monofosforanem adenozyny (agaroza 3′,5′-cAMP) może być stosowana w chromatografii powinowactwa do oddzielania podjednostek kinazy białkowej zależnej od cAMP lub do oczyszczania podjednostki receptora cAMP. Badania nad schizofrenią wykazały, że nieprawidłowości w szlaku sygnałowym cAMP mogą przyczyniać się do patofizjologii tego zaburzenia.
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To better understand the molecular mechanism of cAMP-induced and substrate-enhanced activation of type-I A-kinase, we measured the kinetics of A-kinase regulatory subunit interactions using a stopped-flow spectrofluorometric method. Specifically, we conjugated fluorescein maleimide (FM) to two separate single cysteine-substituted and
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 37(4), 896-905 (2011-11-04)
Recent evidence suggests that schizophrenia may result from alterations of integration of signaling mediated by multiple neurotransmitter systems. Abnormalities of associated intracellular signaling pathways may contribute to the pathophysiology of schizophrenia. Proteins and phospho-proteins comprising mitogen activated protein kinase (MAPK)
Journal of bacteriology, 183(18), 5248-5256 (2001-08-22)
Klebsiella pneumoniae is able to grow anaerobically with citrate as a sole carbon and energy source by a fermentative pathway involving the Na(+)-dependent citrate carrier CitS, citrate lyase, and oxaloacetate decarboxylase. The corresponding genes are organized in the divergent citC
The Journal of biological chemistry, 269(38), 23722-23730 (1994-09-23)
cAMP-dependent protein kinase (PKA) is an important participant in neuronal modulation: the ability of neurons to change their properties in response to external stimuli. In Aplysia mechanosensory neurons, PKA plays roles in both short and long term presynaptic facilitation, which
The soluble protein fraction of tobacco bright yellow 2 cells contained adenosine 3',5'-cyclic monophosphate (cAMP)-binding activity, detected with both a conventional binding assay and a surface plasmon resonance biosensor. A cAMP-agarose-based affinity purification procedure yielded three proteins which were identified
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.