940822104

CHI 034 human cell line

IHW Number: 9200

• Kod UNSPSC: 12352207

Właściwości

• pochodzenie biologiczne: human blood
• Postać: liquid
• pochodzenie etniczne: Thai
• tryb wzrostu: Suspension
• kariotyp: Not specified
• morfologia: Lymphoblastoid
• Warunki transportu: dry ice

Opis

Opis ogólny

Consanguineous: No

Homozygous: No

Opis linii komórkowej

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Profil DNA

HLA DRB1: 1001 11
HLA DRB3: 202
HLA DQAP: 1.3.4.1
HLA DQA1: 0501 0104
HLA DQB1: 0501 0301
HLA DPB1: 02012 3201

Profil serologiczny

HLA A: 11 19
HLA B: 7 40

pożywka hodowlana

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Rutyna subkultury

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Inne uwagi

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable