D-Ribose 5-phosphate is used as a substrate to identify, differentiate and characterize ribose-5-phosphate isomerase(s) and phosphopentomutase(s). Ribose 5-phosphate is used to study the processes and effects of glycation in vivo and in vitro.
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For the synthesis of 5-phospho-D-ribosyl α-1-pyrophosphate[1]; Isolation of transketolase[2]
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European journal of biochemistry, 180(1), 213-219 (1989-03-01)
Transketolase has been purified for the first time from human leukocytes, according to a new procedure which consists of three conventional steps. The enzyme was finally detached from CM-cellulose by specific elution with a D-xylulose-5-phosphate/D-ribose-5-phosphate mixture and the isolated product
Cisplatin is a mainstay of cancer chemotherapy. It forms DNA adducts, thereby activating poly(ADP-ribose) polymerases (PARPs) to initiate DNA repair. The PARP substrate NAD+ is synthesized from 5-phosphoribose-1-pyrophosphate (PRPP), and we found that treating cells for 6 h with cisplatin reduced
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