About This Item
Kod UNSPSC:
41106509
pochodzenie biologiczne
human
metoda przeprogramowania
episomal
opis
age (N/A)
producent / nazwa handlowa
EBiSC™
płeć
male
tryb wzrostu
adherent (pluripotent)
metody
cell culture | stem cell: suitable
powiązane choroby
Alzheimer′s disease
Warunki transportu
dry ice
temp. przechowywania
−196°C
Opis ogólny
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Pochodzenie linii komórkowej
Depositor
Bioneer
Bioneer
Opis linii komórkowej
Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: Unknown
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Morphology:
TREM2 KO cl. 24-54 D1 thaw aft_ekU7aDe.tif - Phase contrast image 1 day after thawing
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Morphology:
BIONi010-C-17 ecto D5 10x.tiff
Protocol or reference:
BIONi010-C-17 ecto D5.tif - Flow cytometry of in vitro differentiation to ectoderm
Endoderm:
Endoderm
In vitro directed differentiation
Morphology:
BIONi010-C-17 endo D5 10x.tiff
Protocol or reference:
BIONi010-C-17 endo D5.tif - Flow cytometry of in vitro differentiation to endoderm
Mesoderm:
Mesoderm
In vitro directed differentiation
Morphology:
BIONi010-C-17 meso D5 10x.tiff
Protocol or reference:
BIONi010-C-17 meso D5.tif - Flow cytometry of in vitro differentiation to mesoderm
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 35
Cell line karyotype: 46, XY
Karyotyping method: G-Banding
Image / Result
Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimer′s disease
Type of modification: Gene knock-out
Gene: TREM2
Chromosome location: KO of exon 1 of TREM2 Transcript variant 1 NM_018965
Delivery method: CRISPR-associated (CRISPR/Cas) System
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Is the reprogramming vector detectable: Unknown
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Morphology:
TREM2 KO cl. 24-54 D1 thaw aft_ekU7aDe.tif - Phase contrast image 1 day after thawing
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Morphology:
BIONi010-C-17 ecto D5 10x.tiff
Protocol or reference:
BIONi010-C-17 ecto D5.tif - Flow cytometry of in vitro differentiation to ectoderm
Endoderm:
Endoderm
In vitro directed differentiation
Morphology:
BIONi010-C-17 endo D5 10x.tiff
Protocol or reference:
BIONi010-C-17 endo D5.tif - Flow cytometry of in vitro differentiation to endoderm
Mesoderm:
Mesoderm
In vitro directed differentiation
Morphology:
BIONi010-C-17 meso D5 10x.tiff
Protocol or reference:
BIONi010-C-17 meso D5.tif - Flow cytometry of in vitro differentiation to mesoderm
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 35
Cell line karyotype: 46, XY
Karyotyping method: G-Banding
Image / Result
Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimer′s disease
Type of modification: Gene knock-out
Gene: TREM2
Chromosome location: KO of exon 1 of TREM2 Transcript variant 1 NM_018965
Delivery method: CRISPR-associated (CRISPR/Cas) System
Powiązanie
Note: EAUA and CLIP must be completed before order fulfillment
Rutyna subkultury
Surface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®
Passage Number banked (pre-EBiSC): 35
No. Vials banked (pre-EBiSC): 30
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: Essential E8®
Passage Number banked (pre-EBiSC): 35
No. Vials banked (pre-EBiSC): 30
Informacje prawne
E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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