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Merck

41367

Sigma-Aldrich

Anti-Rabbit IgG−Abberior® STAR 580 antibody produced in goat

Synonim(y):

Abberior® STAR 580-Anti-Rabbit IgG antibody produced in goat

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About This Item

Kod UNSPSC:
12352200
NACRES:
NA.45

pochodzenie biologiczne

goat

Poziom jakości

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

secondary antibodies

klon

polyclonal

Postać

buffered aqueous solution

reaktywność gatunkowa

rabbit

stężenie

~1 mg/mL

fluorescencja

λex 587 nm; λem 607 nm in PBS, pH 7.4

temp. przechowywania

−20°C

Opis ogólny

Abberior STAR 580 was developed for STED and confocal microscopy in the orange spectral region. Abberior STAR 580 is highly photostable and bright orange fluorescent dye. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with excitation wavelength between 550 - 600 nm. Abberior STAR 580 can substitute dyes like Cy3, Atto 590 or Alexa Fluor 568. For STED microscopy, Abberior STAR 580 can be used most efficiently with a STED Laser wavelength between 750 - 800 nm. Abberior STAR 580 is the ideal partner for Abberior STAR RED or Abberior STAR 635P to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 587 (PBS pH 7.4), 584 (H2O), 584 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 85 000 (PBS pH 7.4), 85 000 (H2O), 95 000 (MeOH)
Correction Factor, CF260 = ε260max: 0,17 (PBS pH 7.4)
Correction Factor, CF280 = ε280max: 0,17 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 607 (PBS pH 7.4), 607 (H2O), 604 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,5 (PBS pH 7.4)

Przydatność

Designed and tested for fluorescent super-resolution microscopy

Komentarz do analizy

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Informacje prawne

ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy3 is a trademark of Cytiva
abberior is a registered trademark of Abberior GmbH
This page may contain text that has been machine translated.

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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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