This product does not contain Fetal Bovine Serum or VEGF. The complete fomulation of this medium is considered proprietary.
211-500
Endothelial Cell Growth Medium (500 ml)
Synonim(y):
Primary Cell Culture Media
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1 EA
930,00 zł
930,00 zł
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Informacje o tej pozycji
NACRES:
NA.71
UNSPSC Code:
12352207
Przejdź do
Pomoc techniczna
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Pozwól nam pomócform
liquid
shelf life
6 mo.
manufacturer/tradename
Cell Applications, Inc
shipped in
wet ice
storage temp.
2-8°C
Quality Level
1 of 4
Ta pozycja | |||
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| shelf life 6 mo. | shelf life - | shelf life - | shelf life 6 mo. |
| storage temp. 2-8°C | storage temp. - | storage temp. - | storage temp. 2-8°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| Quality Level 100 | Quality Level - | Quality Level - | Quality Level 100 |
| manufacturer/tradename Cell Applications, Inc | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename Cell Applications, Inc |
General description
All-in-one ready-to-use media
Endothelial Cell Growth Medium (500 ml) is an all-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies.
Application
Preparation Note
contained in protocol
Analysis Note
Each lot was tested for the ability to support plating, spreading and proliferation
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Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Produkty
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Protokoły
Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.
Technical information for working with human pulmonary artery endothelial cells including thawing, subculturing and cryopreservation
Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol. Storage. Preparation for Culturing. Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Protokół hodowli ludzkich komórek śródbłonka żyły pępowinowej (HUVEC). Przechowywanie. Przygotowanie do hodowli. Przechowywać kriofiole w zbiorniku z ciekłym azotem natychmiast po dostarczeniu.
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does this medium contain FBS? if so, can u specify the concentration?
1 answer-
Helpful?
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Does the medium contain VEGF? If so, can you specify the concentration?
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This medium does not contain VEGF.
Helpful?
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If I use this medium, do I need to use tissue coated treated flasks with gelatin? Does this medium need to be supplemented with heparin or is it already included?
1 answer-
This medium is a complete, ready-to-use product; no supplementation is required. This formulation contains hydrocortisone, heparin and other growth factors needed to support Endothelial Cell growth. Unless a suspension culture is required, the culture vessel should be tissue culture treated. The additional use of attachment matrices such as gelatin, would be dependent on the requirements of the specific cell line or type.
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Can you please provide the full composition of the medium with the concentrations?
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This item is a product of Cell Applications. The formulation is considered proprietary. However, this medium does include glucose at 1g/L, 2% Fetal Bovine Serum, as well as Penicillin, streptomycin, and Amphotericin B. Some salt components have also been published in the Safety Data Sheet.
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Do you sell glucose-free ECGM? I needed it for HUVEC cells. 0mM glucose.
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This product is not a glucose-free medium. The formulation contains 5.78 mM glucose which is 5.55 mM after all the supplements are added. Unfortunately, a glucose-free ECGM medium is not currently offered.
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