This medium is a complete, ready-to-use product; no supplementation is required. This formulation contains hydrocortisone, heparin and other growth factors needed to support Endothelial Cell growth. Unless a suspension culture is required, the culture vessel should be tissue culture treated. The additional use of attachment matrices such as gelatin, would be dependent on the requirements of the specific cell line or type.
211-500
Endothelial Cell Growth Medium (500 ml)
Synonim(y):
Primary Cell Culture Media
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About This Item
Kod UNSPSC:
12352207
NACRES:
NA.71
930,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Polecane produkty
Formularz
liquid
Poziom jakości
okres trwałości
6 mo.
producent / nazwa handlowa
Cell Applications, Inc
Warunki transportu
wet ice
temp. przechowywania
2-8°C
Opis ogólny
All-in-one ready-to-use media
Endothelial Cell Growth Medium (500 ml) is an all-in-one ready-to-use. Optimized and best-suited for angiogenesis and other physiological studies.
Zastosowanie
Jakość
Each lot was tested for the ability to support plating, spreading and proliferation
Uwaga dotycząca przygotowania
contained in protocol
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Certyfikaty analizy (CoA)
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Produkty
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Protokoły
Technical information for working with human pulmonary artery endothelial cells including thawing, subculturing and cryopreservation
Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.
Human Umbilical Vein Endothelial Cells (HUVEC) Culture Protocol. Storage. Preparation for Culturing. Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.
Protokół hodowli ludzkich komórek śródbłonka żyły pępowinowej (HUVEC). Przechowywanie. Przygotowanie do hodowli. Przechowywać kriofiole w zbiorniku z ciekłym azotem natychmiast po dostarczeniu.
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If I use this medium, do I need to use tissue coated treated flasks with gelatin? Does this medium need to be supplemented with heparin or is it already included?
1 answer-
Helpful?
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Can you please provide the full composition of the medium with the concentrations?
1 answer-
This item is a product of Cell Applications. The formulation is considered proprietary. However, this medium does include glucose at 1g/L, 2% Fetal Bovine Serum, as well as Penicillin, streptomycin, and Amphotericin B. Some salt components have also been published in the Safety Data Sheet.
Helpful?
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Do you sell glucose-free ECGM? I needed it for HUVEC cells. 0mM glucose.
1 answer-
This product is not a glucose-free medium. The formulation contains 5.78 mM glucose which is 5.55 mM after all the supplements are added. Unfortunately, a glucose-free ECGM medium is not currently offered.
Helpful?
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