114367

Bromocresol Green sodium salt

90% (HPLC), ACS reagent, Dye content 90 %

• Synonim(y): 3′,3″,5′,5″-Tetrabromo-m-cresolsulfonphthalein sodium salt
• Wzór empiryczny (zapis Hilla): C₂₁H₁₃Br₄NaO₅S
• Numer CAS: 62625-32-5
• Masa cząsteczkowa: 720.00
• NACRES: NA.47
• PubChem Substance ID: 24847167
• UNSPSC Code: 12171500
• EC Number: 263-657-4
• MDL number: MFCD00148898

Właściwości

• grade: ACS reagent
• Quality Level: 200
• agency: suitable for SM 2310, suitable for SM 2320
• assay: 90% (HPLC)
• form: powder or crystals
• composition: Dye content, 90%
• visual transition interval: 3.8, yellow(Visual Interval Pass), 3.8-5.2, yellow to blue, 4.5, green, 5.4, blue(Visual Interval Pass)
• mp: 230 °C (dec.) (lit.)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp

Opis

General description

Bromocresol Green (BCG), also known as bromcresol green, is a synthetic, anionic, triphenylmethane dye belonging to the phthalein and sulphonphthalein dyes group. It is commonly used as a pH indicator as it changes color from yellow to blue-green at pH 3.8-5.4. The monosodium salt of bromocresol green is highly ionizable in water. The dye at a higher pH level produces a large and weakly lipophilic dianion.

Application

Bromocresol green serves as a pH indicator in biology and medicinal experiments.

• It is used widely as a vital stain to study blood-brain permeability.
• It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes.
• It is used in TLC for the visualization of compounds with functional groups whose pKa is below 5.0.
• It has been used in the pH-sensitive colorimetric determination of glutamate decarboxylase activity.
• It has also been employed in the evaluation of paper-analytical devices for colorimetric analysis of serum samples.
• It is suitable as a selective inhibitor in kidney physiology studies.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)