114359

Bromocresol Green

ACS reagent, Dye content 95 %

• Synonim(y): BCG; 2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxy-2-methylphenyl)-1,1-dioxo-2,1λ6-benzoxathiol-3-yl]-3-methylphenol
• Wzór empiryczny (zapis Hilla): C₂₁H₁₄Br₄O₅S
• Numer CAS: 76-60-8
• Masa cząsteczkowa: 698.01
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 24847166
• EC Number: 200-972-8
• Beilstein/REAXYS Number: 372527
• MDL number: MFCD00005874

Właściwości

• grade: ACS reagent
• Quality Segment: 200
• assay: ≥95% (After Oxygen Combustion, silver nitrate titration)
• form: powder
• composition: Dye content, 95%
• visual transition interval: 3.8, yellow(passes test), 3.8-5.4, yellow to blue(passes test), 4.5, green, 5.4, blue
• mp: 225 °C (dec.) (lit.)
• λmax: 423 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cc1c(Br)c(O)c(Br)cc1C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4C
• InChI: 1S/C21H14Br4O5S/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21/h3-8,26-27H,1-2H3
• InChI key: FRPHFZCDPYBUAU-UHFFFAOYSA-N

Opis

General description

Bromocresol Green is a synthetic triphenylmethane dye that is anionic in nature. At pH 3.8-5.4, it changes color from yellow to blue-green, and is therefore, commonly used as a pH indicator.

Application

Bromocresol green serves as a pH indicator in biology and medicinal experiments.

• It is used widely as a vital stain to study blood-brain permeability.
• It has been used in the pH-sensitive colorimetric determination of glutamate decarboxylase activity.
• It has also been employed in the evaluation of paper-analytical devices for colorimetric analysis of serum samples.
• It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes.
• It is used in TLC for the visualization of compounds with functional groups whose pKa is below 5.0.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): Eyeshields, Gloves, type N95 (US)