ERT-RO
Roche
Expand™ Reverse Transcriptase
solution, recombinant, expressed in E. coli (AP401 (k)), sufficient for 20 reactions, sufficient for 100 reactions
Synonim(y):
reverse transcriptase, expand
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Polecane produkty
rekombinowane
expressed in E. coli (AP401 (k))
Postać
solution
zastosowanie
sufficient for 100 reactions
sufficient for 20 reactions
opakowanie
pkg of 1,000 U (11785826001 [20 μl])
pkg of 5,000 U (11785834001 [100 μl])
producent / nazwa handlowa
Roche
Parametry
42-43 °C optimum reaction temp.
metody
RT-PCR: suitable
RT-qPCR: suitable
moc wejściowa
purified RNA
metoda wykrywania
probe-based
temp. przechowywania
−20°C
Opis ogólny
Expand™ Reverse Transcriptase is a RNA directed DNA polymerase optimized for amplification of cDNA fragments up to 13.5 kb in a two-step RT-PCR assay. The enzyme is a genetically engineered version of the Moloney Murine Leukemia Virus reverse transcriptase (M-MuLV-RT). A point mutation within the RNase H sequence reduces RNase H activity to undetectable levels. Detectable improvements are obtained with respect to higher amounts of full-length cDNA transcripts and longer transcripts compared to using the native M-MuLV-RT.
Specyficzność
Heat inactivation: 95 °C for 2 min
Reverse Transcriptase enzyme for RT-PCR reactions up to 3 kb
Reverse Transcriptase enzyme for RT-PCR reactions up to 3 kb
Zastosowanie
Expand™ Reverse Transcriptase has been used in real-time PCR analysis for conversion of isolated RNA into cDNA. It has also been used in reverse transcription (RT). Expand™ Reverse Transcriptase may also be used for analyzing the sequence of the cDNA and comparing it to the genomic sequence. Important information can be obtained on intervening sequences (introns) and genomic recombinations.
Cechy i korzyści
- Obtain higher amounts of full-length cDNA transcripts and longer transcripts up to 13.5kb
- No RNase H activity
- Efficiently transcribes total RNA, mRNA, viral RNA and RNA rich in secondary structures
- Ideal for two-Step RT-PCR assay
- Purification of cDNA before PCR reaction is not necessary
Opakowanie
1 set containing 3 components
Definicja jednostki
One unit is the enzyme activity that incorporates 1.0 nmol TMP into acid insoluble products in 10 min at +37 °C with poly(A)+ × (dT)15 as substrate.
Volume Activity: 50 U/μl
Volume Activity: 50 U/μl
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
Informacje prawne
Expand is a trademark of Roche
Tylko elementy zestawu
Numer produktu
Opis
- Expand Reverse Transcriptase 50 U/μl
- Expand Reverse Transcriptase Buffer 5x concentrated
- DTT Stock Solution 100 mM
Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°C)
does not flash
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
Edwin Yunhao Gong et al.
Methods in molecular biology (Clifton, N.J.), 1030, 195-203 (2013-07-04)
We have developed a duplex real-time RT-PCR assay for profiling antiviral inhibitors of four dengue virus (DENV) serotypes. In this assay, the primers and the probe for amplifying DENV were designed in the conserved regions of the genome after aligned
M Hatzfeld et al.
The Journal of cell biology, 149(1), 209-222 (2000-04-04)
Plakophilin 1, a member of the armadillo multigene family, is a protein with dual localization in the nucleus and in desmosomes. To elucidate its role in desmosome assembly and regulation, we have analyzed its localization and binding partners in vivo.
Kathrin Ikels et al.
PloS one, 9(8), e105349-e105349 (2014-08-22)
The metabolic syndrome is a worldwide problem mainly caused by obesity. FTO was found to be a obesity-risk gene in humans and FTO deficiency in mice led to reduction in adipose tissue. Thus, FTO is an important factor for the
André Spychala et al.
PloS one, 14(2), e0211937-e0211937 (2019-02-08)
Initially, the function of the fat mass and obesity associated (Fto) gene seemed to be primarily the regulation of the body weight. Here we show that loss of Fto results in a hyperactivation of the hypothalamic-pituitary-adrenal (HPA) axis. In consequence
Christoph Gerhardt et al.
PloS one, 8(2), e57545-e57545 (2013-03-08)
Ventricular septal defects (VSDs) are the most common congenital heart defects in humans. Despite several studies of the molecular mechanisms involved in ventricular septum (VS) development, very little is known about VS-forming signaling. We observed perimembranous and muscular VSDs in
Powiązane treści
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
RT-qPCR wykrywa określone cele z zastosowaniami w ekspresji genów i wykrywaniu patogenów.
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej