MAMIC5S10

Multiscreen^® 96 well Plate, polycarbonate membrane

pore size 5 μm, sterile

• Synonym(s): MultiScreen PC Plate, Polycarbonate 96-Well Plate
• UNSPSC Code: 41104923
• NACRES: NB.22
• eCl@ss: 32039006
• Material: clear polystyrene plate, flat bottom wells, polycarbonate membrane
• Pore size: 5 μm pore size
• Sterility: sterile
• Binding type: Tissue Culture (TC)-treated surface

Properties

• Quality Segment: 400
• material: clear polystyrene plate, flat bottom wells, polycarbonate membrane
• description: Clear, Sterile, 5.0 µm pore size, Polycarbonate (PC) membrane, 10 plates
• sterility: sterile
• usage: Manual
• feature: hydrophilic, lid
• packaging: pkg of 10 plates
• manufacturer/tradename: MultiScreen^®
• technique(s): DNA purification: suitable, cell based assay: suitable, cell migration: suitable
• filtration area: 0.28 cm²
• plate size: 96 wells
• well maximum volume: 350 μL
• working volume: 50-250 μL
• pore size: 5 μm pore size
• binding type: Tissue Culture (TC)-treated surface
• compatibility: manifold only
• shipped in: ambient
• storage temp.: 10-30°C

Description

General description

The MultiScreen^®-MIC (Migration, Invasion, Chemotaxis) plate is a 96-well disposable device for use in functional assays for screening protein libraries and compound leads for drug discovery. It is designed to support high throughput cell-based functional assays such as migration, invasion, and chemotaxis with suspension and/or adherent cell lines, using a single device. The plates can be used to perform and quantify cell migration or invasion across coated or uncoated membranes in response to a concentration gradient. The MultiScreen-MIC plates are available in several pore sizes to accommodate MIC assays with a variety of cell lines and are intended for single use only.

Application

MultiScreen^® Migration Invasion and Chemotaxis Filter Plate, 96 well has been used in the transwell/cellular migration assay using:

• interleukin (IL)-4- and IL-12-treated T cells[1]
• THP-1 (human acute monocytic leukemia cell line)[2]
• in vitro activated, purified and IL-2-maintained galectin-3-deficient (gal3)-/- and gal3+/+ cluster of differentiation 4 (CD4)+ OTII T cells[3]

Legal Information

MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany