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MAB5404

Anti-Nitrotyrosine Antibody, clone 2A8.2

Name: Anti-Nitrotyrosine Antibody, clone 2A8.2
Brand: MM

clone 2A8.2, Chemicon^®, from mouse

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Informacje o tej pozycji

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Clone:

2A8.2, monoclonal

Technique(s):

immunohistochemistry: suitable (paraffin), western blot: suitable

Application:

IHC, WB

Citations:

Pomoc techniczna

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Pozwól nam pomóc

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2A8.2, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon^®

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

isotype

IgG2b

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NOS1(4842)

General description

The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.

Immunogen

Nitrated KLH.

Application

Detect Nitrotyrosine using this Anti-Nitrotyrosine Antibody, clone 2A8.2 validated for use in IH(P) & WB.

Immunohistochemistry:
1:50-1:100 on paraffin embedded of a previous lot, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the MAB5404 was for 10 minutes and the reaction was developed with the Chemicon IHC-Select, HRP-DAB kit (Catalog number DAB150).

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Oxidative Stress

Biochem/physiol Actions

Reacts with nitrotyrosine.

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal IgG2b liquid in buffer containing 0.1% sodium azide.

Preparation Note

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Nitrated BSA

Evaluated by Western Blot on Nitrotyrosine Immunoblot Controls.

Western Blot Analysis:
1:500 dilution of this antibody detected Nitrotyrosine on 10 μg of Nitrotyrosine Immunoblot Controls (cat. # 12-354).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Odwiedź Bibliotekę dokumentów

Peroxynitrite decreases arrhythmias induced by ischaemia reperfusion in anaesthetized dogs, without involving mitochondrial KATP channels.

A Kiss et al.

British journal of pharmacology, 155(7), 1015-1024 (2008-10-11)

Exogenous peroxynitrite from nanomolar to micromolar concentrations exerts cardioprotection. Here, we have assessed its effects on ischaemia- and reperfusion-induced ventricular arrhythmias in vivo and a possible role for mitochondrial K(ATP) channels in these effects, using the channel inhibitor 5-hydroxydecanoate (5-HD).

Safety, pharmacokinetics, and pharmacodynamics of oral omaveloxolone (RTA 408), a synthetic triterpenoid, in a first-in-human trial of patients with advanced solid tumors.

Ben C Creelan et al.

OncoTargets and therapy, 10, 4239-4250 (2017-09-19)

Omaveloxolone is a semisynthetic oleanane triterpenoid that potently activates Nrf2 with subsequent antioxidant function. We conducted a first-in-human Phase I clinical trial (NCT02029729) with the primary objectives to determine the appropriate dose for Phase II studies, characterize pharmacokinetic and pharmacodynamic

Molecular hydrogen alleviates motor deficits and muscle degeneration in mdx mice.

Satoru Hasegawa et al.

Redox report : communications in free radical research, 22(1), 26-34 (2016-02-13)

Duchenne muscular dystrophy (DMD) is a devastating muscle disease caused by a mutation in DMD encoding dystrophin. Oxidative stress accounts for dystrophic muscle pathologies in DMD. We examined the effects of molecular hydrogen in mdx mice, a model animal for

Maternal obesity accelerates rat offspring metabolic ageing in a sex-dependent manner.

Guadalupe L Rodríguez-González et al.

The Journal of physiology, 597(23), 5549-5563 (2019-10-09)

Maternal obesity predisposes to metabolic dysfunction in male and female offspring Maternal high-fat diet consumption prior to and throughout pregnancy and lactation accelerates offspring metabolic ageing in a sex-dependent manner This study provides evidence for programming-ageing interactions ABSTRACT: Human epidemiological

Moderate exercise training provides left ventricular tolerance to acute pressure overload.

Daniel Moreira-Gonçalves et al.

American journal of physiology. Heart and circulatory physiology, 300(3), H1044-H1052 (2010-12-28)

The present study evaluated the impact of moderate exercise training on the cardiac tolerance to acute pressure overload. Male Wistar rats were randomly submitted to exercise training or sedentary lifestyle for 14 wk. At the end of this period, the

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Numer pozycji handlu globalnego

SKU	NUMER GTIN
MAB5404	04053252467530

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