ABT340

Anti-AES Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): Amino-terminal enhancer of split, GRG, ESP1, TLE5, Gp130-associated protein GAM, Protein ESP1, Protein GRG
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human
• metody: immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• numer dostępu UniProt: Q08117
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... TLE5(166)

Opis

Opis ogólny

Amino-terminal enhancer of split (AES), also known as GRG or ESP1 or TLE5 or Gp130-associated protein GAM, is a member of the Groucho/ transducin-like enhancer of split (TLE) family. AES belongs to a group of transcriptional co-repressors that play an important role in neurogenesis, segmentation and sex determination. AES is suggested to be a dominant repressor towards other family members of the TLE family and might be required for the initiation and maintenance of the differentiated state. AES has been shown to inhibit the NF-kappa-B-regulated gene expression. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1). Recent studies suggest that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. The apoptosis of cells transfected with AES and Bit1 implies that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and more specifically – with the Alpha5Beta1 integrin. AES is found predominantly in muscle, heart and placenta. Additionally, in fetal tissues, AES is expressed highly in the heart, lung, kidney, brain and liver.

Specyficzność

This antibody recognizes the C-terminus of AES.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human AES.

Zastosowanie

Anti-AES Antibody is a highly specific rabbit polyclonal antibody, that targets Amino-terminal enhancer of split & has been tested in western blotting, ICC & Immunofluorescence.

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected AES in HeLa cells.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected AES in HeLa cells.

Research Category
Cell Structure

Cell Structure

Research Sub Category
Adhesion (CAMs)

Jakość

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected AES in 15 µg of HEK293 cell lysate.

Opis wartości docelowych

~22 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~22 kDa and ~29 kDa

Postać fizyczna

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
HEK293 cell lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable