ABE290
Anti-TET3
from rabbit, purified by affinity chromatography
Synonim(y):
Methylcytosine dioxygenase TET3
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Wybierz wielkość
100 μG
1700,00 zł
Informacje o tej pozycji
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Przejdź do
Pomoc techniczna
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Pozwól nam pomócpochodzenie biologiczne
rabbit
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
mouse
reaktywność gatunkowa (przewidywana na podstawie homologii)
human (based on 100% sequence homology), rat (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology)
metody
ChIP: suitable (ChIP-seq)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
ambient
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... TET3(200424)
1 of 4
Ta pozycja | ABS463 | MABE1132 | SAB2700730 |
|---|---|---|---|
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form affinity isolated antibody |
| biological source rabbit | biological source rabbit | biological source rat | biological source mouse |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| technique(s) ChIP: suitable (ChIP-seq), immunoprecipitation (IP): suitable, immunocytochemistry: suitable, western blot: suitable | technique(s) western blot: suitable | technique(s) ELISA: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable | technique(s) ChIP: suitable, immunofluorescence: suitable, immunohistochemistry: 1:100-1:1000, immunoprecipitation (IP): suitable, western blot: 500-3000 |
| clone polyclonal | clone polyclonal | clone 9F7, monoclonal | clone GT1462, monoclonal |
Opis ogólny
193.7/179.4 kDa (human isoform Tet3FL/Tet3s), 194.8/180.4/185.1 kDa (mouse isoform Tet3FL/Tet3s/Tet3o), 166.7/77.47 kDa (UniProt human isoform 2/3), 76.26 kDa (UniProt mouse isoform 2) calculated. Note that UniProt isoform 1 (O43151-1/human and Q8BG87-1/mouse) corresponds to isoform Tet3s, not Tet3FL (NM_001287491.1/human and JX946278.1/mouse).
MABE1144/1132/1133 Methylcytosine dioxygenase TET3 (EC 1.14.11.n2; UniProt O43151) is encoded by the TET3 (also known as KIAA0401) gene (Gene ID 200424) in human. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported. Three TET3 spliced isoforms/variants have been reported, the long or full-length isoform Tet3FL and the short isoform Tet3s are upregulated during embryonic stem cell (ESC) differentiation toward the neuronal lineage, while Tet3o is oocyte-specific and not detected in ESCs or in any other cell types or adult mouse tissues tested, indicating a role of Tet3o in paternal genome oxidation in mouse zygotes. Tet3FL and Tet3o contain the entire Tet3s sequence with additional (but different) N-terminal sequence. Only Tet3FL contains an N-terminal CXXC domain that binds to unmethylated CpGs and exhibits highest affinity toward 5caC. Tet3FL is substantially less active than Tet3o and Tet3s, suggesting that the CXXC domain restricts Tet3FL 5mC oxidation capacity.
Immunogen
KLH-conjugated linear peptide corrsponding to an internal sequence within the N-terminal half of human TET3.
Zastosowanie
Anti-TET3, Cat. No. ABE290, is a highly specific rabbit polyclonal antibody that targets methylcytosine dioxygenase TET3 and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated the exogenously expressed mouse TET3-GFP fusion protein from 500 µg lysate of transfected mouse embryonic stem cells (mESCs).
Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected TET3 chromosome enrichment sites by ChIP-seq analysis (10 µg per ChIP) using chromatin preparation from mouse embryonic stem cells (mESCs) transfected to overexpress mouse TET3 (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot immunostained 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized mouse embryonic stem cells (mESCs) transfected to overexpress mouse TET3 oocyte isoform Tet3o (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected the immunoreactivity of TET3 oocyte isoform Tet3o in 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized mouse zygotes (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunoprecipitation Analysis: 10 µg from a representative lot co-immunoprecipitated endogenous OGT with the exogenously overexpressed mouse TET3 from transfected mouse embryonic stem cells (mESCs) by RIME (Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins) (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected TET3 chromosome enrichment sites by ChIP-seq analysis (10 µg per ChIP) using chromatin preparation from mouse embryonic stem cells (mESCs) transfected to overexpress mouse TET3 (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot immunostained 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized mouse embryonic stem cells (mESCs) transfected to overexpress mouse TET3 oocyte isoform Tet3o (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected the immunoreactivity of TET3 oocyte isoform Tet3o in 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized mouse zygotes (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunoprecipitation Analysis: 10 µg from a representative lot co-immunoprecipitated endogenous OGT with the exogenously overexpressed mouse TET3 from transfected mouse embryonic stem cells (mESCs) by RIME (Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins) (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Działania biochem./fizjol.
Immunogen sequence is present in TET3 isoforms Tet3FL, Tet3s, and Tet3o spliced isoforms reported by Jin, S.G., et al. (2016). Cell Rep. 14(3):493-505), as well as all additional human and mouse TET3 spliced isoforms reported by UniProt (O43151, Q8BG87). Note that UniProt isoform 1 (O43151-1/human and Q8BG87-1/mouse) corresponds to isoform Tet3s, not Tet3FL (NM_001287491.1/human and JX946278.1/mouse).
Uwaga dotycząca przygotowania
Stable for 1 year at 2-8°C from date of receipt..
Komentarz do analizy
Evaluated by Western Blotting of mouse TET3 GFP fusion.
Western Blotting Analysis: 2 µg/mL of this antibody detected the exogenously expressed mouse TET3 GFP fusion protein in 10 µg of lysate from transfected mouse embryonic stem cells (mESCs).
Western Blotting Analysis: 2 µg/mL of this antibody detected the exogenously expressed mouse TET3 GFP fusion protein in 10 µg of lysate from transfected mouse embryonic stem cells (mESCs).
Inne uwagi
Concentration: Please refer to lot specific datasheet.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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