ABE25

Anti-acetyl-c-Myc (Lys148) Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, HLHe39, Proto-oncogene c-Myc, Transcription factor p64, c-MYC
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: human, mouse
• reaktywność gatunkowa (przewidywana na podstawie homologii): bovine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), porcine (based on 100% sequence homology), canine (based on 100% sequence homology), rat (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), sheep (based on 100% sequence homology)
• metody: western blot: suitable
• numer dostępu NCBI: NP_002458
• numer dostępu UniProt: P01106
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: acetylation (Lys148)
• informacje o genach: bovine ... Myc(511077) ; chimpanzee ... Myc(464393) ; dog ... Myc(403924) ; human ... MYC(4609) ; mouse ... Myc(17869) ; pig ... Myc(448810) ; rat ... Myc(24577) ; rhesus monkey ... Myc(694626) ; sheep ... Myc(443447)

Opis

Opis ogólny

c-Myc (bHLHe39) is a transcription factor that regulates the expression of multiple genes involved in apoptosis, cell growth, differentiation, and proliferation. Active c-Myc is coupled to the MAX protein via c-Myc’s C-terminal helix-loop-helix leucine zipper (bHLHLZ) domain. The c-Myc-MAX heterodimer also interacts with a number of other transcription-related proteins including TRRAP, p107 and Miz-1, and c-Myc-MAX may indirectly regulate histone acetylases involved in chromatin remodeling. c-Myc is itself acetylated on lysine 323 by GCN5, and on lysine 148 and 157 by p300/CBP. The significance of c-Myc acetylation is still under investigation; however, previous studies have suggested that HAT-dependent acetylation of c-Myc may regulate its turnover and stability. c-Myc may play a role in various cancers such as Burkitt’s lymphoma.

Specyficzność

This antibody recognizes c-Myc acetylated at Lys148 in human and Lys149 in mouse.

Immunogen

Epitope: Acetylated Lys148

KLH-conjugated linear peptide corresponding to human c-Myc acetylated at Lys148.

Zastosowanie

Peptide Inhibition Assay Analysis: 2 µg/mL from a representative lot blocked acetyl-c-Myc (Lys148) in HEK293T cells co-transfected with wildtype c-Myc and P300.

Peptide Inhibition Assay Analysis: A representative lot was used by an independent laboratory in HEK293T cells co-transfected with mouse wildtype c-Myc and P300. (Image courtesy of Dr. Ernest Martinez, Department of Biochemistry, University of California at Riverside.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-acetyl-c-Myc (Lys148) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect acetyl-c-Myc (Lys148) also known as HLHe39, Proto-oncogene c-Myc, Transcription factor p64.

Jakość

Evaluated by Western Blot in transfected HEK293 cell lysates.

Western Blot Analysis: 0.1 µg/mL of this antibody detected c-Myc on 10 µg of transfected HEK293 cell lysates.

Opis wartości docelowych

~58 kDa observed. Uniprot describes two isoforms at 49 and 51 kDa Uncharacterized bands appear at ~13 and ~17 in some lysates.

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Transfected HEK293 cell lysates

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable