About This Item
Kod UNSPSC:
12352203
NACRES:
NA.41
pochodzenie biologiczne
rabbit
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
purified antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
masa cząsteczkowa
calculated mol wt 54.70 kDa
observed mol wt ~54 kDa
reaktywność gatunkowa
human
opakowanie
antibody small pack of 100 μg
metody
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
izotyp
IgG
numer dostępu UniProt
Warunki transportu
dry ice
temp. przechowywania
2-8°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... UPF3A(65110)
Opis ogólny
Regulator of nonsense transcripts 3A (UniProt: Q9H1J1; also known as Nonsense mRNA reducing factor 3A, Up-frameshift suppressor 3 homolog A, hUpf3, UPF3A) is encoded by the UPF3A (also known as RENT3A, UPF3) gene (Gene ID: 65110) in human. UPF3A acts as an inhibitor of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. It is reported to shuttle between the nucleus and the cytoplasm. It recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. It binds spliced mRNA upstream of exon-exon junctions. Mice lacking UPF3A exhibit hyper NMD and show defects in embryogenesis and gametogenesis. In human three isoforms of UPF3A have been described that are produced by alternative splicing. (Ref.: Shum, EY., et al. (2016). Cell. 165(2); 382-395).
Specyficzność
This rabbit polyclonal antibody detects human Regulator of nonsense transcripts 3A. It targets an epitope within 16 amino acids from the C-terminal half.
Immunogen
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Regulator of nonsense transcripts 3A (UPF3A).
Zastosowanie
Quality Control Testing
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected UPF3A in HEK293 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:500 dilution from a representative lot detected UPF3A in human fetal brain tissue lysate.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected UPF3A in human testis and human colon tissue sections.
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected UPF3A in lysate from HeLa cells over expressing UPF3A. (Courtesy of Matthew Kim & Dr. Wilkinson Miles at UCSD).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected UPF3A in HEK293 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:500 dilution from a representative lot detected UPF3A in human fetal brain tissue lysate.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected UPF3A in human testis and human colon tissue sections.
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected UPF3A in lysate from HeLa cells over expressing UPF3A. (Courtesy of Matthew Kim & Dr. Wilkinson Miles at UCSD).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-UPF3A, Cat. No. ABE1878, is a rabbit polyclonal antibody that detects Regulator of nonsense transcripts 3A (UPF3A) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Postać fizyczna
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Przechowywanie i stabilność
Stable for 1 year at +2°C to +8°C from date of receipt.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.
Nie możesz znaleźć właściwego produktu?
Wypróbuj nasz Narzędzie selektora produktów.
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej