ABC506

Anti-FUNDC1 Antibody

serum, from rabbit

• Synonim(y): FUN14 domain-containing protein 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human, rat, mouse
• Application: ICC, IP, WB
• Citations: 5

Właściwości

• biological source: rabbit
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, rat, mouse
• technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_776155
• UniProt accession no.: Q8IVP5
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... FUNDC1(139341)

Opis

General description

FUN14 domain-containing protein 1 (FUNDC1) is an important autophagy partner for mitophagy, the process whereby mitochondria get recycled after damage. FUNDC1 is localized to the outer mitochondrial membrane and widely expressed in all cells and appears to act as an activator of hypoxia-induced mitophagy. FUNDC1 interacts directly with important autophagy markers such as MAP1LC3B and GABARAP. Phosphorylation at Tyr-18 inactivates FUNDC1 mitophagy activation ability, and it is this dephosphorylation following hypoxia that initiates the interaction with MAP1LC3 family proteins and mitophagy. Recent research shows that FUNDC1 expression is controlled via the microRNA miRNA-137, and that this microRNA is critical for mitophagy and mitochondrial recycling.

~17 kDa observed.
Uncharacterized bands may be visitible in some lysates.

Immunogen

Epitope: Sigma transmembrane domain

Recombinant protein corresponding to the Sigma transmembrane domain of Human FUNDC1.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-FUNDC1 Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunoprecipitation for the detection of FUNDC1.

Western Blotting Analysis: A representatitive lot of this antibody was used to detect FUNDC1 in A431, C2C12, HEK293, HeLa, HepG2, L6, Mouse brain, NIH/3T3, PC12, PC3, and RAW264.7 cell lysates. This antibody did not detect FUNDC1 in Human placenta, HUVEC, or Jurkat cell lysates.
Western Blotting Analysis: A representatitive lot of this antibody was used to detect puried FUNDC1 protein from bacteria or HeLa cell lysates (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185).
Immunocytochemistry Analysis: A representatitive lot of this antibody was used to detect FUNDC1 in HeLa cells (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185.
Immunoprecipitation Analysis: A representatitive lot of this antibody was used to co-immunoprecipitate FUNDC1 with endogenous LC3 in HeLa cells that were exposed to normoxic or hypoxic conditions for 24 hrs (Liu, L., et al. (2012) Nature Cell Biology. 14(2):177-185).

Physical form

Rabbit Polyclonal serum with 0.05% sodium azide.

Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected FUNDC1 in 10 µg of HepG2 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable