AB15360

Przeciwciało przeciwko peptydowi związanemu z genem kalcytoniny

serum, Chemicon^®

• Synonim(y): CGRP
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IF, IHC, WB
• Citations: 21

Właściwości

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_001741.2, NM_001033952.1, NM_001033953.1
• UniProt accession no.: P01258, P10092
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CALCA(796)

Opis

General description

Calcitonin gene-related peptide 1 (UniProt: Q99JA0; also known as CGRP1, Alpha-type CGRP, Calcitonin gene-related peptide I (CGRP-I)) is encoded by the Calca (also known as Calc) gene (Gene ID: 12310) in murine species. Calcitonin gene-related peptide 1 is a neuropeptide derived by tissue specific alternative splicing of the CALCA precursor, which also yields calcitonin and katacalcin, followed by proteolytic maturation of the pre-prohormone in the secretory pathway of peptidergic neurons and endocrine cells. It is a member of the calcitonin peptide family and adopts an N-terminal disulfide constrained loop and C-terminal amphipathic α helix typical of class B GPCR ligands, which enables high affinity binding to the heterodimeric CGRP receptor composed of calcitonin receptor like receptor (CLR, a class B GPCR) and receptor activity–modifying protein 1 (RAMP1). With lower affinity it can also signal via CTR:RAMP1 “AMY1 like” complexes, providing receptor redundancy within the calcitonin/adrenomedullin/amylin family. Calcitonin gene-related peptide 1 is widely expressed in small diameter primary sensory neurons of dorsal root and trigeminal ganglia, in central projections within brainstem nuclei, in subsets of enteric, autonomic and other peripheral neurons and in perivascular sensory fibers innervating meningeal, cerebral, coronary and systemic resistance arteries. Subcellularly, Calcitonin gene-related peptide 1 is stored in large dense core vesicles in peripheral and central nerve terminals and is released in a Ca²⁺ dependent manner upon nociceptor activation, where it acts primarily as a secreted paracrine hormone/neurotransmitter rather than as an intracellular protein. Functionally, Calcitonin gene-related peptide 1 is one of the most potent endogenous microvascular vasodilators known. Engagement of CLR:RAMP1 on vascular smooth muscle and endothelial cells activates Gs–adenylyl cyclase signaling, elevates cAMP, mobilizes intracellular Ca²⁺, and triggers nitric oxide production, resulting in relaxation of cerebral, meningeal and systemic arteries and elevation of platelet cAMP. Beyond vasodilation, Calcitonin gene-related peptide 1 modulates nociceptive transmission and central sensitization in the trigeminovascular system, promotes neurogenic inflammation (mast cell degranulation, plasma extravasation), sensitizes NMDA receptors, and activates pro survival and growth factor pathways, suggesting neuroprotective roles in ischemia–reperfusion injury. Calcitonin gene-related peptide 1 also plays a central, causative role in migraine and other trigeminal autonomic cephalalgias.

Immunogen

Syntetyczny peptyd związany z genem kalcytoniny.

Application

Evaluated by Immunohistochemistry in rat spinal cord tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:5,000 dilution from a representative lot detected Calcitonin Gene Related Peptide in rat spinal cord tissue sections.

Immunohistochemia na zamrożonym rdzeniu kręgowym szczura: 1:2 000-1:4 000 przy użyciu zestawu Chemicon IHC-Select Kit z systemem wykrywania HRP-DAB.

Optymalne rozcieńczenia robocze muszą być określone przez użytkownika końcowego.

Przeciwciało przeciwko peptydowi związanemu z genem kalcytoniny wykrywa poziom peptydu związanego z genem kalcytoniny i zostało opublikowane i zatwierdzone do stosowania w IH.

Research Sub Category
CNS Control of Metabolism

Hormones & Receptors

Biochem/physiol Actions

Target molecular weight ~ 15 kDa observed. 14.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Rozpoznaje peptyd związany z genem kalcytoniny (CGRP).

Physical form

Rabbit polyclonal antibody in serum with 0.05% sodium azide.

Surowica królicza. Płyn.

Preparation Note

Przechowywać w temperaturze -20°C w nierozcieńczonych podwielokrotnościach przez okres do 12 miesięcy od daty otrzymania. Unikać powtarzających się cykli zamrażania/rozmrażania.

Other Notes

Zastępuje: AB1971

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable