AB1510

Anti-Beta (β)-Amyloid antibody

CHEMICON^®, rabbit polyclonal

• Synonim(y): Beta-APP, A-beta
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: human
• Application: IHC, WB
• Citations: 15

Właściwości

• Nazwa produktu: Anti-Amyloid β Antibody, aa1-14 h β-amyloid, serum, Chemicon^®
• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_000484.2, NM_201413.1, NM_201414.1
• UniProt accession no.: P05067
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... APP(351)

Opis

Immunogen

Epitope: aa1-14 h beta-amyloid

Synthetic peptide that corresponds to the first 14 amino acids of the N-terminal sequence of beta-amyloid. {DAEFRHDSGYEVHH}

Application

Anti-Amyloid Antibody, β, aa1-14 h β-amyloid detects level of Amyloid & has been published & validated for use in WB, IH.

Immunohistochemistry on formic acid treated histological sections. Suggested working dilution is >1:200. AB1510 will detect both cerebrovascular and core amyloid. Formic acid pretreatment (Vinters et al., 1988) involves deparaffinizing sections, follwed by incubation in a 99% formic acid solution (10-20 min), a tap water rinse (10 min), 0.3% H202 in tris-buffered saline, then 3.0% NGS (30 min) followed by primary antibody.

Immunoblotting of formic acid extracts of brain tissue.

ELISA, direct:≥ 1:1000

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Biochem/physiol Actions

Beta amyloid. Sequence is contained in the Amyloid beta-A4 protein amino acids 672-685 of human APP. The sequence is identical in the following other related proteins:

Cerebral vascular amyloid peptide) (CVAP) (Protease nexin-II) (PN-II) (APPI) (PreA4) [Contains: Soluble APP-alpha (S-APP-alpha); Soluble APP-beta (S-APP-beta); C99; Beta-amyloid protein 42 (Beta-APP42); Beta-amyloid protein 40 (Beta-APP40); C83; P3(42); P3(40); Gamma-CTF(59) (Gamma-secretase C-terminal fragment 59) (Amyloid intracellular domain 59) (AID(59)); Gamma-CTF(57) (Gamma-secretase C-terminal fragment 57).

100% homology in human, pig, sheep, rabbit, bovine, 78% identities (11/14), with 12/14 positives (85%) in rat and mouse beta amyloid containing proteins.

Physical form

Rabbit serum with 0.01% thimerosal.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable