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69671

Millipore

Thrombin, Restriction Grade

Synonim(y):

Thrombin, Restriction Grade

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About This Item

Numer CAS:
9002-04-4
Kod UNSPSC:
12352202
NACRES:
NA.54

pochodzenie biologiczne

human plasma

Poziom jakości

400

Postać

liquid

producent / nazwa handlowa

Novagen®

warunki przechowywania

OK to freeze
avoid repeated freeze/thaw cycles

metody

protein extraction: suitable

przydatność

suitable for additive or modifier in the separation of proteins or peptides

Zastosowanie

life science and biopharma

Warunki transportu

wet ice

temp. przechowywania

−20°C

Opis ogólny

Restriction Grade Thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases. Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Thrombin is supplied as a solution in 50% glycerol and includes 10X Thrombin Cleavage Buffer and a Cleavage Control Protein.

Komponenty

•50 UThrombin

•1 ml10X Thrombin Cleavage Buffer

•2 ml1X Thrombin Dilution/Storage Buffer

•10 µgCleavage Control Protein

Ostrzeżenie

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Definicja jednostki

One unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl reaction containing 20 mM Tris-HCl pH 8.4, 150 mM NaCl, 2.5 mM CaCl₂, 50 µg fusion protein.

Uwaga dotycząca przygotowania

Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Informacje prawne

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
This page may contain text that has been machine translated.

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Jooyoung Park et al.
eLife, 8 (2019-12-20)
The computational design of a symmetric protein homo-oligomer that binds a symmetry-matched small molecule larger than a metal ion has not yet been achieved. We used de novo protein design to create a homo-trimeric protein that binds the C3 symmetric
Christian M Smolko et al.
Scientific reports, 9(1), 19409-19409 (2019-12-21)
Protein kinases are enzymes whose abundance, protein-protein interactions, and posttranslational modifications together determine net signaling activity in cells. Large-scale data on cellular kinase activity are limited, because existing assays are cumbersome, poorly sensitive, low throughput, and restricted to measuring one

Produkty

Cleavage Enzymes for Fusion Protein Purification

Find highly-specific cleavage enzymes for isolating proteins during fusion protein purification including enterokinase, factor Xa, HRV 3C protease, and thrombin.

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