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Luminex ® FLEXMAP 3D® System

Enables multiplexing of up to 500 analytes in a single sample, compatible with 96- and 384-well plates for high-throughput applications.

Synonim(y):

3D System, FLEXMAP 3D, Automated multiplexing system, Biomarker analysis system, Gene expression platform, Genomics research tool, High‑throughput analyzer, Immunoassay analyzer, Multiplex assay platform, Multiplex detection system, Multiplexing instrument, Protein analysis instrument

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Informacje o tej pozycji

UNSPSC Code:
41116133
NACRES:
NB.51
eCl@ss:
32161000

Quality Level

200

manufacturer/tradename

Luminex®

technique(s)

multiplexing: suitable

shipped in

ambient

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Ta pozycja
Z687499ZAFS52150ZAFS52120
Millipore

Millipore

40-014

Luminex ® FLEXMAP 3D® System

3D Biotek 3D perfusion bioreactor system with pump 6 well chamber with PS scaffold inserts, autoclavable, AC/DC input 240 V AC, UK plug

Z687499

3D Biotek 3D perfusion bioreactor system with pump

Milli-Q® CLX Water Purification System Delivers up to 3000 L/day of clinical laboratory reagent water (CLRW)

Milli-Q

ZAFS52150

Milli-Q® CLX Water Purification System

Milli-Q® CLX Water Purification System Delivers up to 2400 L/day of clinical laboratory reagent water (CLRW)

Milli-Q

ZAFS52120

Milli-Q® CLX Water Purification System

manufacturer/tradename

Milliplex®

manufacturer/tradename

3D Biotek

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

multiplexing: suitable

technique(s)

-

technique(s)

-

technique(s)

-

detection method

fluorometric

detection method

-

detection method

-

detection method

-

Quality Level

200

Quality Level

-

Quality Level

EQ3

Quality Level

EQ3

General description

Extended Specification
  • Optics Hardware: Lasers/APDs/PMTs
  • Technology: Flow-based
  • Multiplexing: Up to 500
  • Dynamic Range: ≥4.5 logs (RP1)
  • Read Time: 96-well in ~20 min (up to 144,000 tests/hour); 384-well in ~75 min (up to 153,600 tests/hour)
  • Daily start-up/shut down: ≤30 min
  • Applications: Protein/nucleic acid
  • Automation-Compatible Hardware: Front eject only
  • Reporter Laser: 532 nm (green)
  • IQ/OQ: Yes
  • 21 CFR Compliance: Yes
  • Automation: Yes
  • Data Analysis: xPONENT® basic + Belysa® Curve Fitting Software
  • Plate Format: 96-well and 384-well
  • Footprint: 110 cm x 62 cm x 63 cm
  • Weight: 77.1 kg (170 lbs)
The FLEXMAP 3D® System is a flow‑based multiplexing system powered by Luminex® xMAP® technology. It enables simultaneous analysis of up to 500 analytes from a single sample, delivering fast, accurate, and reproducible results. When combined with MILLIPLEX® assays, it provides a complete solution for protein, cytokine, and nucleic acid detection. This powerful platform helps researchers streamline workflows, conserve samples, and accelerate biomarker discovery and translational research.

Application

Powered by xMAP® technology and MILLIPLEX® assays, researchers can perform multiplexed analysis of proteins, cytokines, and other biomarkers in complex biological samples. This versatile platform supports applications in immunology, oncology, and biomarker discovery, enabling high-throughput profiling and reproducible insights that advance scientific research.

Features and Benefits

  • High multiplexing capacity – analyze up to 500 analytes in a single run.
  • Fast read times – ~20 minutes for 96 well plates, ~75 minutes for 384 well plates.
  • Flexible plate formats – supports both 96 and 384 well plates for scalable throughput.
  • Automation ready – integrates seamlessly with front end automation systems to streamline workflows.
  • Reliable performance – robust dynamic range and reproducible results for confident data analysis.

Legal Information

Luminex is a registered trademark of Luminex Corporation
xPONENT is a registered trademark of Luminex Corporation
FLEXMAP 3D is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
BELYSA is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For Research Use Only. Not for use in diagnostic procedures.
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SKUNUMER GTIN
40-01404053252673665

Questions

1–2 of 2 Questions  

  1. I am planning to create a new xPonent protocol template for my assay using a Cytokine Milliplex kit. Which analysis settings do you recommend: Fit of all standards or Mean of replicates?

    1 answer

    1. We generally use the Mean of Replicates method, although Luminex prefers the Fit of All Standards approach.

      Ultimately, the choice between the two methods is left to the researcher to determine the best option for their SOP. Importantly, this choice does not significantly impact sample results.

      Here's a brief overview of the two methods:
      Fit of all standards: This method determines the standard curve by using each individual standard replicate when calculating the standard curve. For instance, if duplicates of a 7-point standard curve are run, the software will calculate the standard curve using 14 points. This method applies only to quantitative analysis.
      Mean of replicates: This method involves averaging the individual standard replicates when calculating the standard curve. Similarly, this method applies only to quantitative analysis. For instance, if duplicates of a 7-point standard curve are run, the software will calculate the standard curve using 7 averaged points.

      Helpful?

  2. Could you provide details on which wells to use on the FM3D probe height adjustment tool for the 384 and 96-well plates utilized in the Milliplex kits?

    1 answer

    1. To use the 96 well plate, utilize well F12 on the probe height adjustment tool (CN-0298-01). For the 384 well mag plate, which is used in the Human High Sensitivity 384 well kit, use well B7.

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