375228

Hepatocyte Growth Factor, Human, Recombinant, S. frugiperda

• Synonim(y): rhHGF
• Kod UNSPSC: 12352202

Właściwości

• Próba: ≥95% (SDS-PAGE)
• Poziom jakości: 100
• Formularz: lyophilized
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze
• zanieczyszczenia: ≤1 EU/μg endotoxin (EU/μg HGF)
• Warunki transportu: ambient
• temp. przechowywania: −70°C

Opis

Opis ogólny

Recombinant, human hepatocyte growth factor expressed in S. frugiperda insect cells. Pleiotropic growth factor that has also been called scatter factor, hepatopoietin A, and mammary growth factor. Through binding to its high affinity met receptor, HGF has marked and varied effects on hepatocytes and other epithelial and endothelial cell types. HGF-induced proliferation of primary hepatocytes is mediated by activation of PI 3-kinase. Acts as a potent mitogen for mature hepatocytes.

Recombinant, human hepatocyte growth factor expressed in Spodoptera frugiperda insect cells. Pleiotropic growth factor that has also been called scatter factor, hepatopoietin A, and mammary growth factor. Through binding to its high affinity met receptor, HGF has marked and varied effects on hepatocytes and other epithelial and endothelial cell types. HGF-induced proliferation of primary hepatocytes is mediated by activation of PI 3-kinase. Acts as a potent mitogen for mature hepatocytes.

Działania biochem./fizjol.

ED₅₀ = 20-40 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation in an HGF-responsive epithelial cell line, 4MBr-5

Ostrzeżenie

Toxicity: Standard Handling (A)

Postać fizyczna

Lyophilized from a sterile-filtered solution in 20 mM Sodium Phosphate, 0.5 M NaCl, 50 µg BSA/µg HGF, pH 7.0.

Rekonstytucja

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥1 µg/ml in sterile PBS containing ≥0.1% HSA or BSA.

Inne uwagi

Kaido, T., et al. 1996. Biochem. Biophys. Res. Commun. 218, 1.
Kobayashi, Y., et al. 1996. Biochem. Biophys. Res. Commun. 220, 7.
Skouteris, G.G., et al. 1996. Biochem. Biophys. Res. Commun. 218, 229.
Rubin, J.S., et al. 1993. Biochim. Biophys. Acta 1155, 357.
Strain, A.J. 1993. J. Endocrinol. 137, 1. Nakamura, T., et al. 1989. Nature 342, 440.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3