361550

BIO

≥97% (HPLC), solid, GSK-3GSK-3α/β inhibitor, Calbiochem

• Synonim(y): GSK-3 Inhibitor IX, BIO, (2ʹ Z,3ʹ E)-6-Bromoindirubin-3ʹ-oxime, BIO, (2ʹZ,3ʹE)-6-Bromoindirubin-3ʹ-oxime
• Wzór empiryczny (zapis Hilla): C₁₆H₁₀BrN₃O₂
• Numer CAS: 667463-62-9
• Masa cząsteczkowa: 356.17
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD08705318
• Assay: ≥97% (HPLC)
• Form: solid
• Storage condition: OK to freeze, protect from light

Właściwości

• Nazwa produktu: GSK-3 Inhibitor IX, BIO, CAS 667463-62-9, is a cell-permeable, highly potent, selective, reversible, and ATP-competitive inhibitor of GSK-3α/β (IC₅₀ = 5 nM). Maintains self-renewal in human & mouse embryonic stem cells.
• Quality Segment: 100
• assay: ≥97% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: purple
• solubility: DMSO: 5 mg/mL
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: Brc1cc2c(cc1)\C(=C3\Nc4c(cccc4)C\3N=O)\C(=O)N2
• InChI: 1S/C16H10BrN3O2/c17-8-5-6-9-12(7-8)19-16(21)13(9)15-14(20-22)10-3-1-2-4-11(10)18-15/h1-7,14,18H,(H,19,21)/b15-13-
• InChI key: WFRDXNRAUPBBTO-SQFISAMPSA-N

Opis

General description

The small molecule, 6-bromoindirubin-3′-oxime (BIO) serves as a specific inhibitor of glycogen synthase kinase-3 (GSK-3). It can sustain self-renewal and pluripotency in both human and mouse embryonic stem cells (ESCs). Treating ESCs with BIO increases β-catenin activity, suggesting that the activation of canonical Wnt signaling plays a key role in preserving the stem cell characteristics. BIO can also induce adult mammalian cardiomyocytes to dedifferentiate and enter mitosis. In addition to its effects on stem cells, the GSK-3 inhibitor IX (BIO) has the ability to elevate the expression of caspase-3 and caspase-8 proteins, leading to the induction of apoptosis in glioblastoma (GBM) cells. This GSK-3 inhibitor also triggers G2/M cell cycle arrest in GBM cells.

Application

BIO has been used as a GSK-3 inhibitor:

• in aortic valve interstitial cells (VICs) culture to induce osteogenesis in VICs
• to treat VICs to study its effects on RUNX2, GSK-3β, and p-SMAD protein expression
• and a Wnt pathway activator to study the effect of Wnt and bone morphogenetic protein (BMP) pathways on Saos-2 using differentiation markers Dlx5, Runx2 and Msx2

Biochem/physiol Actions

Cell permeable: yes

Product competes with ATP.

Reversible: yes

Packaging

Packaged under inert gas

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Carcinogenic / Teratogenic (D)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3