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Merck

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14-864

Białko PAK-1 PBD (bez agarozy), 300 µg

For use in Affinity Binding Assays, ELISA & G-Protein Assays.

Synonim(y):

Kinaza 1 aktywowana przez p21, kinaza 1 aktywowana przez p21/Cdc42/Rac1

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Gabaryty przesyłkiSKUDostępnośćCena netto
300 μg
Sprawdź dostępność w koszyku
1370,00 zł

Informacje o tej pozycji

UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405
Biological source:
human
Mol wt:
Mw 21 kDa

1370,00 zł


Sprawdź dostępność w koszyku

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biological source

human

Quality Level

mol wt

Mw 21 kDa

manufacturer/tradename

Upstate®

concentration

>50% (Coomassie blue staining, SDS-PAGE), 2 mg/mL

technique(s)

ELISA: suitable, affinity binding assay: suitable (G-protein)

NCBI accession no.

UniProt accession no.

shipped in

wet ice

General description

Product Source: GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1, expressed in E. coli.

Physical form

300 µg of PAK-1 PBD, GST in 150 µL of PBS, pH 7.4, 0.5 mM PMSF, containing 20% glycerol.

Preparation Note

Stable for 1 year at -20C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Analysis Note

Routinely evaluated by G-protein activity assay
Specific Activity: Affinity Precipitation Assay: 10 µg of this lot when added with 10 µL glutathione agarose, precipitated Rac-GTP from 3T3 and A431 cell lysates that were loaded in vitro with GTPγS. GDP treated of 3T3 and A431 lysates served as negative controls. Precipitated Rac-GTP was detected by immunoblot analysis using 1 &micor;g/mL anti-Rac (Catalog # 05-389) and secondary goat anti-mouse HRP antibody (Catalog # 12-349) on a Western Blot.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Klasa składowania

10 - Combustible liquids

wgk

WGK 2


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

A Ros-Baro et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(21), 12050-12055 (2001-10-11)
It has been recently reported that insulin recruits a novel signaling machinery to lipid rafts required for insulin-stimulated GLUT4 translocation [Baumann, A., Ribon, V., Kanzaki, M., Thurmond, D. C., Mora, S., Shigematsu, S., Bickel, P. E., Pessin, J. E. &
A X Bigard et al.
European journal of applied physiology and occupational physiology, 76(5), 421-427 (1997-01-01)
We examined the extent of morphological alterations and the myosin heavy chain (MHC) distribution in the rat soleus muscle after a 4-week period of spontaneous recovery or retraining after hindlimb suspension (HS). Moreover, we tested the hypothesis that dantrolene sodium
Shamsideen A Ojelade et al.
PloS one, 10(9), e0137465-e0137465 (2015-09-15)
Responses to the effects of ethanol are highly conserved across organisms, with reduced responses to the sedating effects of ethanol being predictive of increased risk for human alcohol dependence. Previously, we described that regulators of actin dynamics, such as the
Tudor I Oprea et al.
PloS one, 10(11), e0142182-e0142182 (2015-11-13)
Rho family GTPases (including Rac, Rho and Cdc42) collectively control cell proliferation, adhesion and migration and are of interest as functional therapeutic targets in numerous epithelial cancers. Based on high throughput screening of the Prestwick Chemical Library® and cheminformatics we
V Benard et al.
The Journal of biological chemistry, 274(19), 13198-13204 (1999-05-01)
A major function of Rac2 in neutrophils is the regulation of oxidant production important in bacterial killing. Rac and the related GTPase Cdc42 also regulate the dynamics of the actin cytoskeleton, necessary for leukocyte chemotaxis and phagocytosis of microorganisms. Although

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