14-420

Białko MEK1, nieaktywne, 50 g

Unactive, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.

• Synonim(y): Białko MEK1
• UNSPSC Code: 12352202
• NACRES: NA.41
• eCl@ss: 32160405
• Biological source: human
• Recombinant: expressed in E. coli
• Mol wt: M_w 71 kDa

Właściwości

• biological source: human
• Quality Segment: 100
• recombinant: expressed in E. coli
• mol wt: M_w 71 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_002755.2
• UniProt accession no.: Q02750
• shipped in: dry ice
• Gene Information: human ... MAP2K1(5604)

Opis

General description

Research area: CELL SIGNALING

N-terminal GST, and C-terminal His6-tagged, recombinant full-length human MEK1

Product Source: expressed in E.coli

MEK1, also known as Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1), is a protein kinase located on chromosome 15q22.1-q22.33, consisting of 12 exons and 393 amino acids. The protein is made up of two lobes containing several conserved α-helices and β-strands.

Application

Mitogen-Activated Protein Kinase Kinase 1 has been used in in vitro kinase assays:

• to elucidate the underlying mechanisms involved in the response to Rapidly Accelerated Fibrosarcoma (RAF) inhibitors and the interactions with downstream pathways.
• to investigate the role of Mitogen-Activated Protein Kinase Kinase Kinase 2 (MEKK2) as a MAP3K in the Extracellular Signal-Regulated Kinase (ERK) pathway in osteoblasts and to assess its potential as a therapeutic target for treating Neurofibromatosis Type 1 (NF1).
• to assess the effectiveness of the combined targeted therapy with Dabrafenib and Trametinib in treating non-small cell lung cancers (NSCLCs).

Biochem/physiol Actions

Protein Target: MEK1

Target Sub-Family: STE Mitogen-Activated Protein Kinase Kinase 1 (MEK1) is involved in the RAS-RAF-MEK-ERK signaling pathway. This pathway plays a crucial role in various cellular processes, including apoptosis, cell cycle progression, migration, differentiation, metabolism, and proliferation. One of its important functions is the phosphorylation of specific sites in extracellular signal-regulated kinases (ERKs) Mitogen-Activated Protein Kinase /Extracellular Signal-Regulated Kinase (MAPK3/ERK1 and MAPK1/ERK2). MEK activation is closely associated with stimulating cell growth and transformation processes.

Physical form

glutathione-agarose followed by Ni²⁺/NTA-agarose

Preparation Note

6 months at -70°C

Analysis Note

routinely evaluated by phosphorylation of MBP (Catalog # 13-104) substrate in vitro

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable