1.01301

Auramine O (C.I. 41000)

for microscopy

• Synonim(y): Auramine O (C.I. 41000), Pyoktamin yellow, 4,4′-Bis-(dimethylamino)-benzophenonimide hydrochloride
• Wzór empiryczny (zapis Hilla): C₁₇H₂₂ClN₃
• Numer CAS: 2465-27-2
• Masa cząsteczkowa: 303.83
• Numer MDL: MFCD00012484
• Kod UNSPSC: 41116130
• Numer indeksu EC: 219-567-2

Właściwości

• Poziom jakości: 400
• Formularz: solid
• siła działania: 1000 mg/kg LD₅₀, oral (Rat)
• pH: 6-7 (20 °C, 10 g/L in H₂O)
• mp: 265 °C (decomposition)
• rozpuszczalność: 10 g/L
• gęstość nasypowa: 430 kg/m³
• εmax: 1100-1300 at 431-433 nm at 0.005 g/L (pH 6.0)
• Zastosowanie: diagnostic assay manufacturing; hematology; histology
• temp. przechowywania: 2-30°C
• InChI: 1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
• Klucz InChI: KSCQDDRPFHTIRL-UHFFFAOYSA-N

Opis

Opis ogólny

Auramine O (C.I. 41000)-for microscopy, is a dry dye for fluorescence and can be used acc. to Hagemann-Hermannof for the staining acid-fast bacteria (AFB) in smears and sections of human origin . The mechanism of the fluorescence staining is similar to the classical Ziehl-Neelsen method. The cell wall of AFB has a high proportion of wax and lipids and hence absorbs dyes only very slowly. Once the AFB have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, AFB are termed as acid- and alcohol-fast for staining.
Pretreatment of the specimens with Sputofluol^™ dissolves the bacteria from the surrounding viscid sputum and cell material. Sputofluol^™ also has a disinfectant effect, with the result that any microorganisms that are present are killed off. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Zastosowanie

For microscopy. Staining of mycobacteria according to Hagemann-Hermann, fluorescence staining.
CAS No. 2465-27-2, EC Number 219-567-2.

Komentarz do analizy

Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (buffer pH 6.0)431 - 433 nmSpec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; buffer pH 6.0; calc. on dried substance)1100 - 1300TLC-Testpasses testLoss on drying (110 °C)≤ 5 %Suitability for microscopypasses test

Informacje prawne

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07,GHS08,GHS09
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H302 - H319 - H351 - H411
• Zwroty wskazujące środki ostrożności: P201 - P202 - P273 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Klasyfikacja zagrożeń: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3