07-307
Anti-phospho-STAT1 (Tyr701) Antibody
Upstate®, from rabbit
Synonim(y):
Transcription factor ISGF-3 components p91/p84, signal transducer and activator of transcription 1, signal transducer and activator of transcription 1, 91kD, signal transducer and activator of transcription 1, 91kDa, signal transducer and activator of tr
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
affinity purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
polyclonal
oczyszczone przez
affinity chromatography
reaktywność gatunkowa
mouse, chicken, human, rat
producent / nazwa handlowa
Upstate®
metody
western blot: suitable
izotyp
IgG
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
docelowa modyfikacja potranslacyjna
phosphorylation (pTyr701)
informacje o genach
human ... STAT1(6772)
Opis ogólny
STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT 1, is activated by a number of different ligands, including IFNalpha, IFNgamma, EGF, PDGF and IL6. Phosphorylation of tyrosine 701 is required for STAT 1 dissociation from IFNGR1, homodimerization, and nuclear translocation. Tyrosine 701 phosphorylation impairment results in loss of STAT 1 functions.
Specyficzność
Other species cross-reactivity is unknown.
Phosphorylated STAT1
Immunogen
Epitope: Tyr701
KLH-conjugated synthetic peptide (C-DDPKRTG[pY]IKTE) corresponding to amino acids 694-705 of mouse STAT1, containing a N-terminal cysteine.
Zastosowanie
Anti-phospho-STAT1 (Tyr701) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-STAT1 (Tyr701) also known as Transcription factor ISGF-3 components p91/p84 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Jakość
Routinely evaluated by immunoblot in RIPA lysates from CNTF-treated SK-N-MC cells.
Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected phosphorylated STAT1 in RIPA lysates from CNTF-treated SK-N-MC cells.
Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected phosphorylated STAT1 in RIPA lysates from CNTF-treated SK-N-MC cells.
Opis wartości docelowych
92 kDa
Postać fizyczna
Immunoaffinity Chromatography
Immunoaffinity purified IgG in 0.02 M PBS containing 0.1% Sodium Azide + 50% Glycerol and 5 mg/mL BSA.
Przechowywanie i stabilność
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Komentarz do analizy
Control
Mouse 3T3-L1 adipocytes stimulated with leukemia inhibitory factor (LIF).
Mouse 3T3-L1 adipocytes stimulated with leukemia inhibitory factor (LIF).
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Hepatitis C virus core protein differently regulates the JAK-STAT signaling pathway under interleukin-6 and interferon-gamma stimuli.
Hosui, Atsushi, et al.
The Journal of Biological Chemistry, 278, 28562-28571 (2003)
Donor T-cell alloreactivity against host thymic epithelium limits T-cell development after bone marrow transplantation.
Hauri-Hohl, MM; Keller, MP; Gill, J; Hafen, K; Pachlatko, E; Boulay, T; Peter et al.
Blood null
Porcine reproductive and respiratory syndrome virus Nsp1? inhibits interferon-activated JAK/STAT signal transduction by inducing karyopherin-?1 degradation.
Wang, Rong, et al.
Journal of virology, 87, 5219-5228 (2013)
Precision-cut slice cultures of tumors from MMTV-neu mice for the study of the ex vivo response to cytokines and cytotoxic drugs.
Nirmala Parajuli, Wolfgang Doppler
In Vitro Cellular & Developmental Biology. Animal null
Induction of type I interferons by a novel porcine reproductive and respiratory syndrome virus isolate.
Nan, Yuchen, et al.
Virology, 432, 261-270 (2012)
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