07-1519

Anti-phospho Grb10 (Ser503) Antibody

from rabbit, purified by affinity chromatography

• Synonim(y): Tumor necrosis factor ligand superfamily member 4, OX40 ligand, OX40L, CD252
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: mouse
• reaktywność gatunkowa (przewidywana na podstawie homologii): primate (based on 100% sequence homology), porcine (based on 100% sequence homology), human (immunogen homology)
• metody: inhibition assay: suitable (peptide); western blot: suitable
• numer dostępu NCBI: NP_005302
• numer dostępu UniProt: Q13322
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: phosphorylation (pSer503)
• informacje o genach: human ... GRB10(2887) ; mouse ... Grb10(14783) ; pig ... Grb10(100188977)

Opis

Opis ogólny

Grb10 (insulin receptor-binding protein Grb-IR) is a ubiquitous adapter protein which is expressed in both fetal and adult tissues. It is a multidomain protein that interacts with a wide array of cellular targets; the PH domain allows interaction with several subtypes of phosphoinositides; the Ras-associating domain binds a wide variety of Ras GTPases; and the SH domain facilitates binding to membrane-bound tyrosine kinase receptors which link Grb10 to different signaling pathways. Most notably, Grb10 associates with the insulin receptor at phosphotyrosine residues via its C-terminal BPS (between PH and SH) domain and inhibits the enzymatic activity of the insulin receptor. Grb10 also acts downstream of the insulin receptor pathway to inhibit activation of AKT-1. In addition, Grb10 suppresses Wnt signalling by binding to the low-density lipoprotein receptor. In fetal tissues, Grb10 may inhibit growth and placental size.

Specyficzność

Other homologies: Rat (91% sequence homology).

This antibody recognizes Grb10 phosphorylated at (Ser503).

Immunogen

Epitope: Phosphorylated (Ser503)

KLH-conjugated linear peptide corresponding to human GRB10 phosphorylated at (Ser503).

Zastosowanie

Peptide Inhibition Assay Analysis: 1 µg/mL from a representative lot detected phospho-Grb10 (Ser503) on 10 µg of embryonic fibroblast cell lysate + non-phospho-Grb10 (Ser503) peptide. However, the antibody was inhibited when incubated with a phospho-Grb10 (Ser503) peptide.

Research Category
Signaling

Research Sub Category
Developmental Signaling

This Anti-phospho Grb10 (Ser503) Antibody is validated for use in Peptide Inhibition Assay, Western Blotting for the detection of phospho Grb10 (Ser503).

Jakość

Evaluated by Western Blot in mouse embryonic fibroblast cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected phospho-Grb10 (Ser503) on 10 µg of mouse embryonic fibroblast cell lysate.

Opis wartości docelowych

~75 kDa observed. An uncharacterized band appears at ~16 kDa in some lysates.

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Mouse embryonic fibroblast cell lysate

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable