806005

APN-Amine HCl

95%

• Synonim(y): 3-(4-(4-(Aminomethyl)-1H-1,2,3-triazol-1-yl)phenyl)propiolonitrile hydrochloride
• Wzór empiryczny (zapis Hilla): C₁₂H₁₀N₅Cl
• Masa cząsteczkowa: 259.69
• Kod UNSPSC: 12352200
• Identyfikator substancji w PubChem: 329768982

Właściwości

• Poziom jakości: 100
• Próba: 95%
• Postać: powder
• przydatność reakcji: reagent type: cross-linking reagent
• temp. przechowywania: 2-8°C
• ciąg SMILES: N#CC#CC1=CC=C(N2C=C(CN)N=N2)C=C1.[H]Cl
• InChI: 1S/C12H9N5.ClH/c13-7-1-2-10-3-5-12(6-4-10)17-9-11(8-14)15-16-17;/h3-6,9H,8,14H2;1H
• Klucz InChI: JZGAUVVBWJHLGN-UHFFFAOYSA-N

Opis

Zastosowanie

APN-Amine is a bifunctional crosslinker for thiol-to-carboxyl coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions. The amino group readily reacts with activated carboxylic acids (e.g. NHS-esters) in basic conditions to produce stable amide bonds. The APN group can be used for coupling with free thiols (e.g. labeling of free cysteines in proteins).

Inne uwagi

Standard protein labeling procedure (cysteine labeling):

• Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
• Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
• Incubate at room temperature for 2 hours.
• If necessary, purify the protein conjugate using size exclusion chromatography or ultrafiltration.

*Note: avoid thiol-containing buffers.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable