760706

Sulfo-dibenzocyclooctyne-biotin conjugate

for Copper-free Click Chemistry

• Synonim(y): Sulfo-DBCO-biotin
• Wzór empiryczny (zapis Hilla): C₃₇H₅₀N₆O₇S₂
• Masa cząsteczkowa: 754.96
• MDL number: MFCD22572660
• UNSPSC Code: 12352200
• PubChem Substance ID: 329766890
• NACRES: NA.22
• Form: solid

Właściwości

• Quality Segment: 100
• form: solid
• reaction suitability: reaction type: click chemistry, reagent type: cross-linking reagent
• storage temp.: −20°C
• SMILES string: CC[NH+](CC)CC.[O-]S(=O)(=O)C(CNC(=O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)C(=O)NCCC(=O)N3Cc4ccccc4C#Cc5ccccc35
• InChI: 1S/C31H35N5O7S2.C6H15N/c37-27(12-6-5-11-25-29-23(19-44-25)34-31(40)35-29)33-17-26(45(41,42)43)30(39)32-16-15-28(38)36-18-22-9-2-1-7-20(22)13-14-21-8-3-4-10-24(21)36;1-4-7(5-2)6-3/h1-4,7-10,23,25-26,29H,5-6,11-12,15-19H2,(H,32,39)(H,33,37)(H2,34,35,40)(H,41,42,43);4-6H2,1-3H3/t23-,25-,26?,29-;/m0./s1
• InChI key: RACLEVCKGUBCTH-GESOYTMLSA-N

Opis

General description

This sulfonated azadibenzocyclooctyne-biotin derivative is a highly water soluble and versatile biotinylation reagent for labeling azide containing molecules or compounds. Cyclooctynes are useful in strain-promoted copper-free azide-alkyne cycloaddition reactions.This dibenzocyclooctyne will react with azide-functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage. Subsequent labeled molecules can be purified using streptavidin or avidin affinity reagents.

Application

Automate your Biotin tagging with Synple Automated Synthesis Platform (SYNPLE-SC002)

Features and Benefits

More efficient labeling will occur at higher temperatures and with greater excess of biotin reagent. Typical reaction times are up to 4 hours, but longer incubation periods should be used if concentration is low or if low labeling is observed. Product is most efficient when first dissolved in a water miscible organic solvent and then diluted in aqueous reaction buffer when needed. Stock solutions in DMSO have been shown to be stable in the freezer for up to 3 months.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Słowo ostrzegawcze: Warning
• Kody zagrożeń: H315,H319,H335
• Ostrzeżenia: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Narządy docelowe: Respiratory system
• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable