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199303

Resazurin sodium salt

certified by the BSC

Synonim(y):

7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt

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Rozmiar/SKUDostępnośćCena netto
1 g
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232,00 zł
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Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C12H6NNaO4
Numer CAS:
Masa cząsteczkowa:
251.17
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
263-718-5
Beilstein/REAXYS Number:
3794971
MDL number:

232,00 zł


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agency

certified by the BSC

Quality Segment

form

powder

composition

Dye content, 75%

solubility

1 M NH4OH: 1 mg/mL

λmax

380 nm (2nd), 598 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[O-]c1ccc2c(OC3=CC(=O)C=CC3=[N+]2[O-])c1

InChI

1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1

InChI key

IVGPGQSSDLDOLH-UHFFFAOYSA-M

General description

Resazurin is a small N-oxide derivative of dihydroxyphenoxazone. It is a non-ionic dye containing a small and planar chromophore. It is a redox indicator and changes from bluish violet through pink to colorless upon reduction. It is also used as a pH indicator for Ph 3.5 to 6.5 wherein it changed from orange to bluish violet. It is commercially available as resazurin sodium salt, which is also known as 3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide, sodium salt. It forms the red, fluorescent hydroxyphenoxazone resorufin on reduction and colorless hydroresorufin on further reduction.

Application

For the measurement of metabolic activity and proliferation of living cells. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).
Resazurin is mostly used as a biological redox indicator. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red). Its applications include:
  • the measurement of metabolic activity and the proliferation of living cells
  • the detection of bacterial reduction in milk
  • in viability assays as a component of culture media for the detection of viable bacteria
  • to determine the number of motile sperm in sheep semen
  • to assay lactate dehydrogenase
  • as an analytical reagent to quantify L-glutamate
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Ta pozycja
R7017TOX8A4043
description

certified by the BSC

description

powder, BioReagent

description

-

description

certified by the BSC

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

cell analysis
detection

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

powder

form

liquid

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

2-8°C

storage temp.

room temp

solubility

1 M NH4OH: 1 mg/mL

solubility

H2O: soluble

solubility

-

solubility

H2O: 1 mg/mL

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Dye content, 75%

composition

Dye content, ~80%

composition

-

composition

Dye content, ≥80%


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Piktogramy

Exclamation mark

Słowo ostrzegawcze

Warning

Kody zagrożeń

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Narządy docelowe

Respiratory system

Klasa składowania

11 - Combustible Solids

Co się dzieje?

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

PPE (środki ochrony indywidualnej)

dust mask type N95 (US), Eyeshields, Gloves



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Certyfikaty analizy (CoA)

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Questions

  1. Can you tell me the precise difference between R7017 and 199303? I am interested in exposing live cells to Resazurin salt. Is one of these two products more appropriate for that purpose?

    1 answer
    1. There is very little difference between these two products, based on physical properties. Each product originated from a different brand with the R7017 originating from the Sigma brand while the 199303 was originally an Aldrich brand. In addition, the R7017 has a dye content of ~80% and is listed as a BioReagent, meaning the tests performed are more relevant to molecular biology or cell culture applications. Product 199303 has 75% dye content and is certified by the Biological Stain Commission. These Certified dyes are tested to ensure the quality of dyes through independent testing to meet appropriate chemical and performance criteria. In most application areas, these two products would be interchangeable.

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