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MDA-MB-231 Cell Line human

92020424, human breast, Epithelial

Synonym(s):

MD Anderson-Metastatic Breast-231 Cells, MDA-MB231 Cells, MDA231-BRE Cells, MDAMB231 Cells

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32190102
Pricing and availability is not currently available.

Product Name

MDA-MB-231 Cell Line human, human breast adenocarcinoma, 92020424

biological source

human breast

growth mode

Adherent

karyotype

Modal no.′s 62 and 64, near triploid

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian breast adenocarcinoma

Cell Line Description

Isolated from pleural effusions of a breast cancer patient.

Application

MDA-MB-231 has been used to study:
  • the changes in the morphological pattern and gene expression of cells when grown in the presence of sera positive for anti-carbonic anhydrase I (CA I) autoantibodies[1]
  • the effects of normothermic conditioned microwave irradiation on cultured cells using an irradiation system[2]
  • the transendothelial migration rates of the cells under different shear stress levels[3]

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12,13
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 8,9
THO1: 7,9.3
TPOX: 8,9
vWA: 15,18

Culture Medium

L15+ 2mM Glutamine + 15% Foetal Bovine SerumFBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e. seeding at 3-6x10,000 cells/cm2 using 0.05% Trypsin or Trypsin/EDTA; 37°C. CO2 is not required.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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Articles

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

Questions

1–9 of 9 Questions  

  1. Gent.mi, il codice prodotto si riferisce alla linea cellulare o al DNA estratto da essa? Grazie

    1 answer

    1. Product 92020424-1VL is for the cells themselves, MDA-MB-231 Cell Line human. Product 92020424-DNA-5UG is for the corresponding DNA.

      Helpful?

  2. Doubts about complete growth media that I have to use with MDA-MB-231. Is it L15 + 2 mM Glutamine+10% FBS or Can I use DMEM, 10% FBS, PenStrep? Protocol that I have to follow after thawing cells (volume, rpm, V to resuspend, FlaskT25 or T75 and ratio)

    1 answer

    1. It is best to adhere to the guidelines recommended by ECACC, at least for bringing cells out of the freeze. Some end users have successfully transitioned cells to other media, but this should always be a gradual transition over several sub-cultures to ensure robust cell growth and morphology.

      For this cell line see this list of products that will meet the supplier's requirements:
      L-15 Leibovitz Medium – L1518 Sterile Liquid or L4386 Powdered Medium (with L-Glutamine)
      https://www.sigmaaldrich.com/product/sigma/l1518
      https://www.sigmaaldrich.com/product/sigma/l4386

      FBS – F2442
      https://www.sigmaaldrich.com/product/sigma/f2442

      Trypsin – T4424
      https://www.sigmaaldrich.com/product/sigma/t4424

      Trypsin EDTA – T4049
      https://www.sigmaaldrich.com/product/sigma/t4049

      The traditionalists advise avoiding using antibiotics, however, if impeccable aseptic technique is a challenge and busy labs and incubators are common, antibiotics are acceptable. Should the added assurance of Pen/Strep be preferred, see product P0781:
      https://www.sigmaaldrich.com/product/sigma/p0781

      The T25 flasks would be best for starting up cultures. After the culture is established, end users may prefer the T75 flasks, provided there is adequate cell density.

      See the link below to the supplier product information for additional information and details:
      https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=92020424&collection=ecacc_gc

      Helpful?

  3. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 answer

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

      Helpful?

  4. How many Cell Line cells are in the vial?

    1 answer

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

      Helpful?

  5. What medium should I use when I thaw the Cell Line cells?

    1 answer

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

      Helpful?

  6. What passage are my Cell Line cells?

    1 answer

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

      Helpful?

  7. Are the Cell Line cells tested for viral pathogens?

    1 answer

    1. HPA Cultures does not perform any viral testing on the cell lines.

      Helpful?

  8. Are my Cell Line cells mycoplasma tested?

    1 answer

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

      Helpful?

  9. How do I handle Cell Line frozen cells upon arrival?

    1 answer

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

      Helpful?

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