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C6788

4-Chloro-1-naphthol

peroxidase substrate, tablet, suitable for use in immunoblotting procedures

Synonym(s):

4-CN, 4-chloronaphthalen-1-ol

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5 tabs
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PLN 236.00
50 tabs
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PLN 1,260.00
100 tabs
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PLN 2,190.00
PLN 1,861.50

About This Item

Linear Formula:
ClC10H6OH
CAS Number:
604-44-4
Molecular Weight:
178.61
NACRES:
NA.83
PubChem Substance ID:
57654035
UNSPSC Code:
12352204
EC Number:
210-068-5
MDL number:
MFCD00003974
Beilstein/REAXYS Number:
509750

PLN 236.00

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Product Name

4-Chloro-1-naphthol, tablet

form

tablet

Quality Segment

200

mp

118-121 °C (lit.)

solubility

methanol: 10 mL, clear, colorless

storage temp.

−20°C

SMILES string

Oc1ccc(Cl)c2ccccc12

InChI

1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H

InChI key

LVSPDZAGCBEQAV-UHFFFAOYSA-N

Application

4-Chloro-1-naphthol has been used as a substrate in enzyme-linked immunospot (ELISpot) assay for detection of the spots.[1] It has also been used as a precipitating chromogenic substrate for visualizing the antigen−antibody-reactive bands following western blotting.

Biochem/physiol Actions

4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.

Preparation Note

Contains 30 mg substrate per tablet.
Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use.

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This Item
C8890N2765N2640
4-Chloro-1-naphthol tablet

Sigma-Aldrich

C6788

4-Chloro-1-naphthol

4-Chloro-1-naphthol crystalline

Sigma-Aldrich

C8890

4-Chloro-1-naphthol

4-Nitrophenyl phosphate disodium salt hexahydrate tablet

Sigma-Aldrich

N2765

4-Nitrophenyl phosphate disodium salt hexahydrate

4-Nitrophenyl phosphate disodium salt hexahydrate tablet

Sigma-Aldrich

N2640

4-Nitrophenyl phosphate disodium salt hexahydrate

solubility

methanol: 10 mL, clear, colorless

solubility

acetone: 50 mg/mL, clear, colorless to very faintly brown

solubility

water, high purity: 0.25 tablet/mL, clear, colorless

solubility

water: 1 tablet/3 mL, clear, colorless

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

form

tablet

form

crystalline

form

tablet

form

tablet

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mp

118-121 °C (lit.)

mp

118-121 °C (lit.)

mp

-

mp

-

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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