N2640

4-Nitrophenyl phosphate disodium salt hexahydrate

chromogenic, tablet

• Synonym(s): p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate
• Linear Formula: O₂NC₆H₄OP(O)(ONa)₂ · 6H₂O
• CAS Number: 333338-18-4
• Molecular Weight: 371.14
• NACRES: NA.83
• PubChem Substance ID: 24897580
• UNSPSC Code: 12352204
• EC Number: 224-246-5
• MDL number: MFCD00066288
• Beilstein/REAXYS Number: 5324661

Properties

• Product Name: 4-Nitrophenyl phosphate disodium salt hexahydrate, tablet
• form: tablet
• Quality Segment: 200
• solubility: water: 1 tablet/3 mL, clear, colorless
• storage temp.: −20°C
• SMILES string: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• InChI key: KAKKHKRHCKCAGH-UHFFFAOYSA-L

Description

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used in enzyme-linked immunosorbent assay (ELISA) as a substrate for streptavidin-Alkaline Phosphatase. It has also been used as a substrate in control assay for comparing 4-aminophenyl phosphate/diethanolamine (APP/DEA) assay for alkaline phosphatase.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.

Preparation Note

Contains 15 mg substrate per tablet

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)