A8111

Plasminogen activator inhibitor 1 (PAI-1)

≥200,000 units/mg protein, lyophilized powder, ≥90% (SDS-PAGE)

• Synonym(s): PAIE, PLANH1, Plasminogen activator inhibitor, β-migrating endothelial cell-derived type, Serpin E1 PAI-1, Serpine 1
• CAS Number: 140208-23-7
• UNSPSC Code: 12352200
• NACRES: NA.32
• MDL number: MFCD01094930

Properties

• Product Name: Plasminogen activator inhibitor 1 (PAI-1) human, recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
• recombinant: expressed in E. coli
• Quality Segment: 200
• assay: ≥90% (SDS-PAGE)
• form: lyophilized powder
• specific activity: ≥200,000 units/mg protein
• mol wt: ~43 kDa
• solubility: water: 0.1 mL, clear, colorless
• UniProt accession no.: P05121
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... SERPINE1(5054)

Description

General description

Plasminogen activator inhibitor 1 (PAI-1) or serine protease inhibitor E1 (SERPINE1) gene is mapped to human chromosome 7q22.1.[1] PAI-1 is produced in hepatocytes, endothelial cells, smooth muscle cells, megakaryocytes, and adipocytes.[2]

Application

Plasminogen activator inhibitor 1 (PAI-1) human has been used:

• as an inhibitor of pro-brain-derived neurotrophic factor (proBDNF) in radioimmunoprecipitation assay (RIPA) in peripheral blood and lymphocytes[3]
• to block plasmin activation in mice infected with C. albicans hyphae[4]
• to test its effect on neurite growth in neurons[5]

Biochem/physiol Actions

Plasminogen Activator Inhibitor 1 (PAI-1) protein belongs to the Serpin superfamily of serine protease inhibitors. It is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and therefore inhibits the fibrinolysis process. PAI is involved in extracellular matrix remodeling and has been implicated in processes like angiogenesis, chemotaxis, ovulation, and embryogenesis. PAI-1 is present in increased levels in various disease states such as a number of cancer forms, obesity and the metabolic syndrome.

Plasminogen activator inhibitor 1 (PAI-1), a member of the Serpin superfamily of serine protease inhibitors, is involved in extracellular matrix remodeling and implicated in processes like angiogenesis, chemotaxis, ovulation, and embryogenesis.

Plasminogen activator inhibitor–1 (PAI -1) participates in mediating cellular senescence.[6] It is upregulated during injury along with other tissue repair transcriptomes.[7] Elevated levels of PAI-1 is regarded as one of the risks associated with myocardial infarction (MI).[2]

Physical form

Supplied as a lyophilized powder from a solution containing 0.5 M sodium chloride, 20 mM sodium acetate, and 50 mg/mL trehalose.

Preparation Note

Reconstitute to 0.25 mg/ml with ultrapure water. The reconstituted product contains 0.25 mgP/mL in 0.5 M sodium chloride, 20 mM sodium acetate, and 50 mg/mL trehalose.

Analysis Note

The product is the active human PAI-1 protein with a molecular weight of 43 kDa.

Other Notes

1 unit irreversibly inhibits (100% inhibition) 1 unit of tissue plasminogen activator (tPA) under physiological conditions (neutral pH and 37 ºC).

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable