MABC004F

Mouse IgG2a Negative Control, clone GC270, FITC conjugate

Mouse IgG2a Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

• UNSPSC Code: 12352203
• NACRES: NA.42
• eCl@ss: 32160702
• Conjugate: FITC conjugate
• Clone: GC270, monoclonal
• Application: FACS, IF
• Citations: 3

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: FITC conjugate
• antibody form: ascites fluid
• clone: GC270, monoclonal
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunofluorescence: suitable
• isotype: IgG2a
• shipped in: wet ice
• target post-translational modification: unmodified

Description

Immunogen

Protein I preparation from Neisseria gonorrhoeae.

Application

Fluorescein conjugated mouse IgG2a is a monoclonal antibody intended for use as a negative control in immunofluorescence, flow cytometry. Its use enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.

SUGGESTED USAGE

Flow cytometry and immunofluorescence - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) or bone marrow cells in 100 μl buffer.

Mouse IgG2a Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

Research Category
Secondary & Control Antibodies

Epitope Tags & General Use

Research Sub Category
Isotype Control Antibodies

Biochem/physiol Actions

This antibody reacts with the outer membrane protein of some strains of Neisseria gonorrhoeae. No reaction with human cell surface or plasma components has been observed. However, some binding to Fc receptors may occur.

Physical form

Purified from mouse ascitic fluid. Fluorescein conjugated, ready to use. The antibody is supplied in 1.0ml phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.

Preparation Note

Store at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable