MABC004

Mouse IgG2a Negative Control, clone GC270

• UNSPSC Code: 12352203
• NACRES: NA.42
• eCl@ss: 32160702
• Clone: GC270, monoclonal
• Technique(s): flow cytometry: suitable, immunofluorescence: suitable
• Application: FACS, IF
• Citations: 6

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• clone: GC270, monoclonal
• manufacturer/tradename: Chemicon^®
• concentration: 0.5 mg/mL
• technique(s): flow cytometry: suitable, immunofluorescence: suitable
• isotype: IgG2a
• shipped in: wet ice
• target post-translational modification: unmodified

Description

Immunogen

Protein I preparation from Neisseria gonorrhoeae.

Application

Mouse IgG2a is a monoclonal antibody intended for use as a negative control in immunofluorescence, flow cytometry, and immunoperoxidase staining on frozen and paraffin tissue sections. Its use enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.

SUGGESTED USAGE

The control antibody should be diluted to the same concentration as the test antibody, and equivalent volumes used.

Research Category
Secondary & Control Antibodies

Epitope Tags & General Use

Research Sub Category
Isotype Control Antibodies

Biochem/physiol Actions

This antibody reacts with the outer membrane protein of some strains of Neisseria gonorrhoeae. No reaction with human cell surface or plasma components has been observed. However, some binding to Fc receptors may occur.

Physical form

Format: Purified

Purified from mouse ascitic fluid. The antibody is supplied in 0.5ml 25mM Tris-HCl, 0.4M NaCl, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis, specific immunofluorescence assay and flow cytometry.

Preparation Note

Store at 2 to 8°C, for up to 18 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Other Notes

Replaces: CBL601

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• wgk: WGK 1
• flash_point_f: not combustible
• flash_point_c: not combustible