For cellular staining using SSEA-1 in flow cytometry, prepare an antibody working solution by diluting the primary antibody 1:5 with PBS. Dispense the volume per test of the working solution according to the number of cells indicated below:
- Use 5 µL for Guava Flow Cytometer with 5x10^5 cells in 95 µL PBS, totaling 100 µL.
- Use 10 µL for other flow cytometry systems with 1x10^6 cells in 90 µL PBS, totaling 100 µL.
FCMAB117P
Anti-SSEA-1 Antibody, clone MC-480, PE conjugate
clone MC-480, from mouse, PE
Synonym(s):
SSEA-1
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100 TESTS
PLN 1,400.00
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100 TESTS
PLN 1,400.00
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
PE
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MC-480, monoclonal
species reactivity
rat, mouse, human
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgM
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... FUT4(2526)
Related Categories
General description
SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
Specificity
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
Immunogen
Epitope: MC-480
F9 tetracarcinoma stem cells (X-irradiated).
Application
Anti-SSEA-1 Antibody, clone MC-480, PE conjugate is an antibody against SSEA-1 for use in IH, IP, IF, FC.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
Quality
Evaluated by Flow Cytometry with mouse embryonic stem cells.
Target description
220 kDa Calculated
Physical form
Purified mouse monoclonal IgM conjugated to PE fluorochrome in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage and Stability
Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.
Analysis Note
Control
Mouse embryonic Stem Cells
Mouse embryonic Stem Cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Suggest suitable dilaution of SSEA-1 for flow cutometry.
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