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MAB4360

Sigma-Aldrich

Anti-TRA-1-60 Antibody, clone TRA-1-60

clone TRA-1-60, Chemicon®, from mouse

Synonym(s):

TRA-1-60 Marker

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

TRA-1-60, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

input

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

isotype

IgM

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PODXL(5420)

General description

Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.

Specificity

This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.

Immunogen

Human embryonal carcinoma cell line 2102Ep

Application

Immunofluorescence: A previous lot was used in IF.

Western Blot: A previous lot of this antibody was used in WB.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunohistochemistry: A previous lot of this antibody was used in IH.

Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-TRA-1-60 Antibody, clone TRA-1-60 is validated for use in WB, FC, IF, IP, IC for the detection of TRA-1-60.

Target description

235/410 kDa

Physical form

Format: Purified
Purified mouse monoclonal IgM liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide.

Storage and Stability

Stable for 1 year at from date of receipt.

Analysis Note

Control
NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Glycan stem-cell markers are specifically expressed by spermatogonia in the adult non-human primate testis.
Muller, T; Eildermann, K; Dhir, R; Schlatt, S; Behr, R
Human Reproduction null
Mahdieh Jadaliha et al.
PloS one, 7(6), e38532-e38532 (2012-06-23)
Analysis of gene expression to define molecular mechanisms and pathways involved in human embryonic stem cells (hESCs) proliferation and differentiations has allowed for further deciphering of the self-renewal and pluripotency characteristics of hESC. Proteins associated with hESCs were discovered through
Tomoko Andoh-Noda et al.
Molecular brain, 8, 31-31 (2015-05-28)
Rett syndrome (RTT) is one of the most prevalent neurodevelopmental disorders in females, caused by de novo mutations in the X-linked methyl CpG-binding protein 2 gene, MECP2. Although abnormal regulation of neuronal genes due to mutant MeCP2 is thought to
Livia Eiselleova et al.
The International journal of developmental biology, 52(4), 353-363 (2008-04-17)
Various types of feeder cells have been adopted for the culture of human embryonic stem cells (hESCs) to improve their attachment and provide them with stemness-supporting factors. However, feeder cells differ in their capacity to support the growth of undifferentiated
Sriram Bandi et al.
Journal of cell science, 125(Pt 5), 1274-1283 (2012-02-22)
Understanding the identity of lineage-specific cells arising during manipulations of stem cells is necessary for developing their potential applications. For instance, replacement of crucial functions in organ failure by transplantation of suitable stem-cell-derived cells will be applicable to numerous disorders
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