With HEPES, without ʟ-glutamine, ʟ-leucine, ʟ-lysine, ʟ-methionine, CaCl2, MgCl₂, MgSO4, sodium bicarbonate, and phenol red, powde, suitable for cell cutlure
Synonym(s):
Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham, DME/F-12, 1:1 mixture
DMEM/Nutrient Mixture F-12 Ham, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: no HEPES: 15 mM sodium pyruvate: 0.055 g/L NaHCO3: no phenol red: no glucose: 3.15 g/L
The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of (1;1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with labeL-tagged amino acids.
Quantity
Formulated to contain 14.8 grams of powder per liter of medium.
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