D2906

DMEM/F-12

With ʟ-glutamine, sodium pyruvate and HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

• Synonym(s): DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture
• MDL number: MFCD00217417
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• product name: Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture
• Quality Level: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: NaHCO₃: no; L-glutamine: yes; phenol red: no; sodium pyruvate: 0.055 g/L; glucose: 3.15 g/L; HEPES: 15 mM
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

• Hep-2 cells
• mouse fibroblast L‐cells
• L‐ Wnt family member 3A (Wnt3a) cells
• primary buffalo embryonic fibroblast (BEF)

Quantity

Formulated to contain 15.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1