Skip to Content
Merck
All Photos(1)

Key Documents

D0547

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

DME/F-12, 1:1 mixture

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.71

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

  • to culture cerebral cortices from mice embryos
  • as a component of the human corneal epithelium (HCE) media for culturing human limbal explants
  • to culture human corneal epithelial cell lines

Other Notes

Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate

Quantity

Formulated to contain 12.0 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service