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S2015

Sigma-Aldrich

SSPE Buffer 20× Concentrate

for Northern and Southern blotting, solution

Sinónimos:

20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer

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About This Item

Código UNSPSC:
41105319
NACRES:
NA.25

grado

for molecular biology

Nivel de calidad

200

esterilidad

0.2 μm filtered

Formulario

solution

actividad extraña

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

temp. de almacenamiento

room temp

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Descripción general

SSPE Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.

Aplicación

Suitable for:
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for microarrays

Características y beneficios

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Componentes

SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000406274
0000405184
0000395525
0000302806
0000355824

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Visite la Librería de documentos

Warren P Pickering et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 18(8), 1475-1484 (2003-08-05)
Increased protein degradation during metabolic acidosis contributes to muscle wasting in uraemia. Adrenalectomy experiments in severely acidotic rats (arterial pH approximately 7.15) have shown that this is prevented in the absence of glucocorticoid. It should therefore be possible to block
Glucocorticoid antagonist RU38486 fails to block acid-induced muscle wasting in vivo or in vitro.
Pickering WP, et al.
Nephrology, Dialysis, and Transplantation, 18, 1475-1484 (2003)
Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures.
Pierce SE, et al.
Nature Protocols, 2, 2958-2974 (2007)
Claudia S Meier-Trummer et al.
PloS one, 4(7), e6265-e6265 (2009-07-16)
Malignant catarrhal fever (MCF) is a lethal disease of cattle, characterized by vasculitis, necrosis, and accumulation of activated, dysregulated cytotoxic lymphocytes in various tissues. Ovine gamma herpesvirus 2 (OvHV-2) is a causative agent of MCF, which may trigger the disease

Protocolos

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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