This product has historically been synthetically produced. However, it is best to refer to the lot-specific Certificate of Origin, as the method of manufacture and origin of future lots may be subject to change. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under the ‘Certificate of Origin’ tab or click on the link below to request a lot-specific Certificate of Origin:
https://www.sigmaaldrich.com/product/sigma/d0428#product-documentation
D0428
1 kb DNA Ladder
for DNA electrophoresis
Sinónimos:
1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, DNA gel marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker
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Acerca de este artículo
NACRES:
NA.25
UNSPSC Code:
41105335
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Servicio técnico
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liquid
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis, five μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR™ Green, and Nancy-520.
Application
Other Notes
For optimal resolution, the recommended agarose gel concentration is 0.75%.
Sigma′s 1kb Ladder is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Legal Information
SYBR is a trademark of Thermo Fisher Scientific or its subsidiaries
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Este artículo | |||
|---|---|---|---|
| description for DNA electrophoresis | description ready-to-use marker for DNA electrophoresis | description ready-to-use marker for DNA electrophoresis | description - |
| form liquid | form liquid | form liquid | form solid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability - |
NA electrophoresis | ladder, marker, NA electrophoresis | ladder, marker, NA electrophoresis | - |
Clase de almacenamiento
10 - Combustible liquids
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
EPI (equipos de protección individual)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Does this product have biological origin? Or is the DNA synthetically formulated?
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Helpful?
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how to use this product. in what ratio we have to add dye in this.
1 answer-
This product has been tested successfully at 2 µL, 5µL, and 7 µL., however for general use, a starting volume of 5 µL is recommended. Dilute 5 µL volume of D0428 with 25 µL of gel loading buffer. Product G2526 is used for testing purposes. For best results, a 0.75% gel is recommended.
See the link below to Gel Loading Buffer, G2526:
https://www.sigmaaldrich.com/product/sigma/g2526Helpful?
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