One potential replacement is item number P7405. This is the HBr form, making it more water-soluble. However, it may be necessary to conduct tests to evaluate its compatibility with your specific cell line. If needed, there are methods to prepare the solution and subsequently remove the HBr through dialysis. This polymer is of the same size (>300,000), gamma-irradiated, and has been tested for cell culture.
P7405
Poly-ᴅ-Lysine Hydrobromide
synthetic, mol wt >300,000, powder, γ-irradiated, suitable for cell culture, BioXtra
Sinónimos:
PDL
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About This Item
Fórmula lineal:
D-Lys-(D-Lys)n-D-Lys · xHBr
Número de CAS:
MDL number:
UNSPSC Code:
12352202
PubChem Substance ID:
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
Nombre del producto
Poli-D-lisina hydrobromide, mol wt >300,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture
biological source
synthetic (chemical)
Quality Level
sterility
γ-irradiated
product line
BioReagent
BioXtra
form
lyophilized powder
mol wt
>300,000
packaging
pkg of 5 mg
concentration
0.016—0.032 mmol lysine
technique(s)
cell culture | mammalian: suitable
surface coverage
4 μg/cm2
solubility
H2O: soluble 50 mg/mL, clear, colorless
shipped in
ambient
storage temp.
−20°C
SMILES string
O=C(C)[C@@](NC)([H])CCCCN.[Br]
InChI
1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H
InChI key
MEXAGTSTSPYCEP-UHFFFAOYSA-N
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General description
Poly-D-Lysine Hydrobromide (PDL) is a cationic polymer composed of lysine residues with an associated hydrobromide molecule per lysine unit. The presence of the hydrobromide molecule introduces a distinctive property to the poly-D-lysine structure allowing the poly-D-lysine to adopt a crystalline form, rendering it soluble in aqueous solutions.
Application
Los polímeros de poli-D-lisina pueden utilizarse en la preparación de las superficies para fijación celular. Los polímeros de D-lisina también pueden utilizarse con células que digieren los polímeros poli-L-lisina y causan un captación excesiva de L-lisina.
Este producto está recomendado como sustrato de cultivo celular cuando se utilizan entre 0,5 y 1,0 ml de una disolución 0,1 mg/ml para recubrir 25 cm2. Las versiones del producto de menor peso molecular son menos viscosas, pero las versiones de gran peso molecular proporcionan mas sitios de fijación por molécula.
Este producto está recomendado como sustrato de cultivo celular cuando se utilizan entre 0,5 y 1,0 ml de una disolución 0,1 mg/ml para recubrir 25 cm2. Las versiones del producto de menor peso molecular son menos viscosas, pero las versiones de gran peso molecular proporcionan mas sitios de fijación por molécula.
Poly-D-lysine hydrobromide has been used :
- in preparing the surface of coverslips for cell attachment[1] [2]
- in coating glass-bottom dishes for live cell imaging of primary cortical neuronal cells[3]
- in coating 24-well plates for the isolation of primary astrocytes to study the impact of melanoma-derived exosomes on the reprogramming of stromal cells within the metastatic microenvironment[4]
- for coating 16-well chamber slides to study the effects of maternal immune activation on behavioral impairments and alterations in cytokine and synaptic protein expression in the cerebellum[5]
Biochem/physiol Actions
El hidrobromuro de poli-D-lisina es un factor de unión inespecífica para las células, útil en la promoción de la adhesión celular a los sustratos sólidos al intensificar la interacción electrostática entre iones con carga negativa de la membrana celular y la superficie del cultivo. Después de la absorción a la superficie del cultivo, la poli-D-lisina aumenta el número de sitios de unión a la célula con carga positiva.
Poly-D-Lysine Hydrobromide (PDL) is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.[6] PDL increases the number of positively charged cell binding sites post-absorption to the culture surface.[7] PDL coating promotes the attachment of cells to solid surfaces that usually grow in suspension.[8]
Components
La poli-D-lisina es una polímero de aminoácido con carga positiva con aproximadamente un HBr por residuo de lisina. El hidrobromuro permite a la poli-D-lisina estar en una forma cristalina hidrosoluble. Puede encontrarse una pequeña cantidad del producto en la estructura ß porque el HBr interfiere en el enlace de hidrógeno entre el grupo amino y los grupos carboxilo o los grupos que contienen N u O.
Caution
Las disoluciones estériles son estables durante un máximo de 2 años cuando se conservan entre 2 y 8°C. Deben guardarse desecadas a -20°C.
Analysis Note
This product has a molecular weight >300,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Los clientes también vieron
On the interaction of polystyrene latices and poly(l-lysine): II. Characterization of the particle-polymer System
Corry W.D
Journal of Colloid and Interface Science, 63(1), 151-160 (1978)
Monitoring Scavenging Activity of Chemokine Receptors
Barbara Moepps, et al.
Methods in Enzymology, 570, 87-118 (2016)
Direct addition of poly-lysine or poly-ethylenimine to the medium: A simple alternative to plate pre-coating
Alexander Faussner
PLoS ONE, 17(7), e0260173-e0260173 (2022)
CRISPR-Mediated Activation of ?V Integrin Subtypes Promotes Neuronal Differentiation of Neuroblastoma Neuro2a Cells
Riccardi S, et al.
Frontiers in genome editing, 4 (2022)
Maternal Immune Activation Causes Behavioral Impairments and Altered Cerebellar Cytokine and Synaptic Protein Expression
Pendyala G, et al.
Neuropsychopharmacology, 42, 1435?1446-1435?1446 (2017)
Artículos
Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.
Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.
Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.
Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.
Protocolos
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.
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Have you considered any alternative products that have been tested and found suitable for the primary culture of cortical neurons? This inquiry arises as the expected delivery date for the previously ordered A-003-E Poly-D-lysine has been postponed to April 2022, and the current stock is expected to be depleted soon.
1 answer-
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